Characterization of Human Colon Carcinoma Cell Lines Isolated from a Single Primary Tumour

Overview

Journal Br J Cancer

Specialty Oncology

Date 1983 Mar 1

PMID 6830688

Citations 18

Authors

M G Brattain

M E Marks

J McCombs

W Finely

D E Brattain

Affiliations

Soon will be listed here.

Abstract

The initiation of a cultured human colon carcinoma line on a feeder layer of confluent fibroblasts is described. Attempts to initiate cultures without fibroblast feeder layers were not successful. Two sub-lines (designated HCT C and HCT C Col) were isolated and weaned from cells growing on the surface of the feeder layer. The sub-lines had different morphologies, secreted different levels of carcinoembryonic antigen (CEA) into the medium of confluent cultures and had slightly different karyotypes. Both sub-lines grew in semi-solid medium and formed xenografts when injected s.c. into athymic nude mice. Analysis of radioiodinated cell membrane components indicated small, but significant differences between the sub-lines.

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