DNA Replication in Short-time Organ Cultures of Human Epidermis. Inhibition by Aphidicolin, and Detection of DNA Polymerases Alpha, Beta, and Gamma

Overview

Journal Arch Dermatol Res

Specialty Dermatology

Date 1982 Jan 1

PMID 6820927

Authors

H Krokan

G Volden

Affiliations

Soon will be listed here.

Abstract

Human epidermis uncontaminated by fibroblasts was isolated by a suction blister method. DNA synthesis in short-time organ cultures of isolated epidermis was strongly inhibited by aphidicolin, suggesting that DNA polymerase alpha is involved in DNA replication in human epidermis. On the basis of their responses to inhibitors, primer-template requirements, and chromatographic properties, DNA polymerases alpha, beta, and gamma were all identified in epidermal extracts.

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