Butyrate Sensitive Suppressor of Position-effect Variegation Mutations in Drosophila Melanogaster

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1982 Jan 1

PMID 6819429

Citations 21

Authors

G Reuter

R Dorn

H J Hoffmann

Affiliations

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Abstract

Mutations at a locus on chromosome II of D. melanogaster suppressing position-effect variegation mutations have been identified which display recessive butyrate sensitivity. Survival of homozygous mutant flies is significantly reduced on medium containing sodium n-butyrate. The butyrate sensitive suppressor mutations are further characterized by recessive female sterility and reduced survival of homozygotes. Complementation analysis showed their allelism. The locus of these mutations, Su-var (2) 1, has been localized to 40.5 +/- 0.2 and, by using interstitial duplications, to region 31CD on the cytogenetic map. Moreover, the mutant alleles of the Su-var (2) 1 locus display a lethal interaction with the heterochromatic Y chromosome. The presence or absence of a Y chromosome in males or females has a strong influence on the viability of homozygous or transheterozygous suppressor flies. All the genetic properties of Su-var (2) 1 mutants suggest strongly that this locus affects chromosome condensation.

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References

Riggs M, Whittaker R, Neumann J, Ingram V . n-Butyrate causes histone modification in HeLa and Friend erythroleukaemia cells. Nature. 1977; 268(5619):462-4. DOI: 10.1038/268462a0. View

Reuter G, Werner W, Hoffmann H . Mutants affecting position-effect heterochromatinization in Drosophila melanogaster. Chromosoma. 1982; 85(4):539-51. DOI: 10.1007/BF00327349. View

Mottus R, Reeves R, Grigliatti T . Butyrate suppression of position-effect variegation in Drosophila melanogaster. Mol Gen Genet. 1980; 178(2):465-9. DOI: 10.1007/BF00270501. View

HARTMANN-GOLDSTEIN I . On the relationship between heterochromatization and variegation in Drosophila, with special reference to temperature-sensitive periods. Genet Res. 1967; 10(2):143-59. DOI: 10.1017/s0016672300010880. View

BAKER W . Position-effect variegation. Adv Genet. 1968; 14:133-69. View

KHESIN R, Leibovitch B . Influence of deficiency of the histone gene-containing 38B-40 region on X-chromosome template activity and the white gene position effect variegation in Drosophila melanogaster. Mol Gen Genet. 1978; 162(3):323-8. DOI: 10.1007/BF00268858. View

LEWIS E . The phenomenon of position effect. Adv Genet. 1950; 3:73-115. DOI: 10.1016/s0065-2660(08)60083-8. View

PROKOFYEVA-BELGOVSKAYA A . Heterochromatisation as a change of chromosome cycle. J Genet. 2010; 48(1):80-98. DOI: 10.1007/BF02986099. View

Sealy L, Chalkley R . The effect of sodium butyrate on histone modification. Cell. 1978; 14(1):115-21. DOI: 10.1016/0092-8674(78)90306-9. View

10.

Candido E, Reeves R, Davie J . Sodium butyrate inhibits histone deacetylation in cultured cells. Cell. 1978; 14(1):105-13. DOI: 10.1016/0092-8674(78)90305-7. View

11.

BLUMENFELD M, Orf J, Sina B, Kreber R, Callahan M, Mullins J . Correlation between phosphorylated H1 histones and satellite DNAs in Drosophila virilis. Proc Natl Acad Sci U S A. 1978; 75(2):866-70. PMC: 411358. DOI: 10.1073/pnas.75.2.866. View

12.

Sandler L . Evidence for a set of closely linked autosomal genes that interact with sex-chromosome heterochromatin in Drosophila melanogaster. Genetics. 1977; 86(3):567-82. PMC: 1213695. DOI: 10.1093/genetics/86.3.567. View

13.

Davie J, Candido E . Acetylated histone H4 is preferentially associated with template-active chromatin. Proc Natl Acad Sci U S A. 1978; 75(8):3574-7. PMC: 392827. DOI: 10.1073/pnas.75.8.3574. View

14.

Ananiev E, Gvozdev V . Changed pattern of transcription and replication in polytene chromosomes of Drosophila melanogaster resulting from eu-heterochromatin rearrangement. Chromosoma. 1974; 45(2):173-91. DOI: 10.1007/BF00362310. View

15.

EPHRUSSI B, Herold J . Studies of Eye Pigments of Drosophila. I. Methods of Extraction and Quantitative Estimation of the Pigment Components. Genetics. 1944; 29(2):148-75. PMC: 1209240. DOI: 10.1093/genetics/29.2.148. View

16.

LINDSLEY D, Sandler L, Baker B, Carpenter A, Denell R, Hall J . Segmental aneuploidy and the genetic gross structure of the Drosophila genome. Genetics. 1972; 71(1):157-84. PMC: 1212769. DOI: 10.1093/genetics/71.1.157. View

17.

Boffa L, Gruss R, Allfrey V . Manifold effects of sodium butyrate on nuclear function. Selective and reversible inhibition of phosphorylation of histones H1 and H2A and impaired methylation of lysine and arginine residues in nuclear protein fractions. J Biol Chem. 1981; 256(18):9612-21. View

18.

Reuter G, Wolff I . Isolation of dominant suppressor mutations for position-effect variegation in Drosophila melanogaster. Mol Gen Genet. 1981; 182(3):516-9. DOI: 10.1007/BF00293947. View

19.

Moore G, Procunier J, Cross D, Grigliatti T . Histone gene deficiencies and position--effect variegation in Drosophila. Nature. 1979; 282(5736):312-4. DOI: 10.1038/282312a0. View