Culture-produced Subendothelium. II. Effect of Plasma, F.VIIIR:WF and Fibronectin on Interaction of Normal Platelets with Normal and Von Willebrand Porcine Aortic Subendothelium

Culture-produced normal and von Willebrand (vWd) porcine aortic subendothelium (SE) was prepared on glass and fibronectin (FN)-coated surfaces. Washed porcine platelets reacted extensively with normal SE (on glass) as single adherent non-spread and spread (5%) platelets, whereas vWd SE (on glass)-platelet interaction was decreased with no spreading. Normal porcine plasma increased normal SE-platelet interaction 3 to 5-fold and spreading 5-fold (20-30%); vWd SE-platelet interaction was increased 2 to 4-fold with no spreading. vWd porcine plasma did not affect normal or vWd SE-platelet interaction or spreading. Purified porcine F.VIIIR:WF (0.5-2 U/ml) increased both normal and vWd SE-platelet interaction 2 to 4-fold without increasing spreading. Purified human FN (50-200 micrograms/ml) did not increase normal of vWd SE-platelet interaction but increased spreading 5 to 7-fold (25-35%) with normal SE. F.VIIIR:WF (2 U/ml) plus FN (200 micrograms/ml) increased normal SE-platelet interaction 4 to 5-fold and spreading 8 to 9-fold (41%) with extensive SE-associated microaggregate formation; vWd SE-platelet interaction was increased about 6-fold with no spreading. Platelets reacted more extensively with normal and vWd SE prepared on FN-coated surfaces than SE on glass. Normal SE (on FN)-platelet interaction increased 6 to 7-fold and spreading 12-fold (60%); vWd SE (on FN)-platelet interaction increased about 110-fold with about 10% spreading. Plasma (normal and vWd), and F.VIIIR:WF did not significantly increase normal or Vwd SE (on FN)-platelet interaction or spreading. Results suggest a possible role for FN as a spreading factor in SE-platelet interactions.