Expression of Ia-like Antigens by Human Vascular Endothelial Cells is Inducible in Vitro: Demonstration by Monoclonal Antibody Binding and Immunoprecipitation

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Nov 1

PMID 6815654

Citations 40

Authors

J S Pober

M A Gimbrone Jr

Affiliations

Soon will be listed here.

Abstract

The expression of Ia-like antigens by cultured human endothelial cells has been investigated by means of monoclonal antibody binding to intact cells and by immunoprecipitation of radioiodinated membrane proteins. Primary growing and confluent cultures of human umbilical vein endothelium express little, if any, detectable Ia-like antigens under standard culture conditions. However, treatment of primary cultures with the lectin phytohemagglutinin induces the expression of Ia-like antigens. This action of the lectin uniformly affects all the endothelial cells in a culture, does not depend on cell division, and is associated with a cell shape change. The data presented in this report provide unequivocal serological and biochemical demonstration of Ia-like antigens on human vascular endothelial cells. The fact that the expression of Ia-like antigens by endothelium can be induced may have important implications for organ transplantation and for regulation of the immunological response.

Citing Articles

Interferon-γ converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1.

Liu R, Merola J, Manes T, Qin L, Tietjen G, Lopez-Giraldez F JCI Insight. 2018; 3(5).

PMID: 29515027 PMC: 5922286. DOI: 10.1172/jci.insight.97881.

Brugia malayi microfilariae adhere to human vascular endothelial cells in a C3-dependent manner.

Schroeder J, McCarthy D, Szestak T, Cook D, Taylor M, Craig A PLoS Negl Trop Dis. 2017; 11(5):e0005592.

PMID: 28481947 PMC: 5436873. DOI: 10.1371/journal.pntd.0005592.

Endothelial Cell Dysfunction and the Pathobiology of Atherosclerosis.

Gimbrone Jr M, Garcia-Cardena G Circ Res. 2016; 118(4):620-36.

PMID: 26892962 PMC: 4762052. DOI: 10.1161/CIRCRESAHA.115.306301.

Modes of Antigen Presentation by Lymph Node Stromal Cells and Their Immunological Implications.

Hirosue S, Dubrot J Front Immunol. 2015; 6:446.

PMID: 26441957 PMC: 4561840. DOI: 10.3389/fimmu.2015.00446.

Endothelial microparticles interact with and support the proliferation of T cells.

Wheway J, Latham S, Combes V, Grau G J Immunol. 2014; 193(7):3378-87.

PMID: 25187656 PMC: 4170003. DOI: 10.4049/jimmunol.1303431.

References

Moen T, Moen M, Thorsby E . HLA-D region products are expressed in endothelial cells. Tissue Antigens. 1980; 15(2):112-22. DOI: 10.1111/j.1399-0039.1980.tb00896.x. View

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Hirschberg H, Evensen S, Henriksen T, Thorsby E . The human mixed lymphocyte-endothelium culture interaction. Transplantation. 1975; 19(6):495-504. DOI: 10.1097/00007890-197506000-00008. View

Kessler S . Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. J Immunol. 1975; 115(6):1617-24. View

Humphreys R, McCune J, Chess L, Herrman H, Malenka D, Mann D . Isolation and immunologic characterization of a human. B-lymphocyte-specific, cell surface antigen. J Exp Med. 1976; 144(1):98-112. PMC: 2190359. DOI: 10.1084/jem.144.1.98. View

Robb R, Strominger J . Rapid purification of detergent-solubilized HLA antigen by affinity chromatography employing anti-beta2-microglobulin serum. J Biol Chem. 1976; 251(17):5427-8. View

Morris R, Williams A . Antigens on mouse and rat lymphocytes recognized by rabbit antiserum against rat brain: the quantitative analysis of a xenogeneic antiserum. Eur J Immunol. 1975; 5(4):274-81. DOI: 10.1002/eji.1830050412. View

Moraes J, Stastny P . A new antigen system expressed in human endothelial cells. J Clin Invest. 1977; 60(2):449-54. PMC: 372387. DOI: 10.1172/JCI108795. View

Barnstable C, Bodmer W, Brown G, Galfre G, Milstein C, Williams A . Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell. 1978; 14(1):9-20. DOI: 10.1016/0092-8674(78)90296-9. View

10.

Hirschberg H, Moen T, Thorsby E . Complement and cell-mediated specific destruction of human endothelial cells treated with anti-DRw antisera. Transplant Proc. 1979; 11(1):776-8. View

11.

Brodsky F, Bodmer W, Parham P . Characterization of a monoclonal anti-beta 2-microglobulin antibody and its use in the genetic and biochemical analysis of major histocompatibility antigens. Eur J Immunol. 1979; 9(7):536-45. DOI: 10.1002/eji.1830090709. View

12.

Koyama K, Fukunishi T, Barcos M, TANIGAKI N, Pressman D . Human Ia-like antigens in non-lymphoid organs. Immunology. 1979; 38(2):333-41. PMC: 1457930. View

13.

Krangel M, Orr H, Strominger J . Assembly and maturation of HLA-A and HLA-B antigens in vivo. Cell. 1979; 18(4):979-91. DOI: 10.1016/0092-8674(79)90210-1. View

14.

Hirschberg H, Bergh O, Thorsby E . Antigen-presenting properties of human vascular endothelial cells. J Exp Med. 1980; 152(2 Pt 2):249s-255s. View

15.

Steinman R, Nogueira N, Witmer M, Tydings J, Mellman I . Lymphokine enhances the expression and synthesis of Ia antigens on cultured mouse peritoneal macrophages. J Exp Med. 1980; 152(5):1248-61. PMC: 2185993. DOI: 10.1084/jem.152.5.1248. View

16.

Thompson J, Overlin V, Severson C, Parsons T, Herbick J, Strauss R . Demonstration of granulocyte, monocyte, and endothelial cell antigens by double fluorochromatic microcytotoxicity testing. Transplant Proc. 1980; 12(3 Suppl 1):26-31. View

17.

Scher M, Beller D, UNANUE E . Demonstration of a soluble mediator that induces exudates rich in Ia-positive macrophages. J Exp Med. 1980; 152(6):1684-98. PMC: 2186030. DOI: 10.1084/jem.152.6.1684. View

18.

Steeg P, Moore R, Oppenheim J . Regulation of murine macrophage Ia-antigen expression by products of activated spleen cells. J Exp Med. 1980; 152(6):1734-44. PMC: 2186042. DOI: 10.1084/jem.152.6.1734. View

19.

Brodsky F, Parham P, Bodmer W . Monoclonal antibodies to HLA--DRw determinants. Tissue Antigens. 1980; 16(1):30-48. DOI: 10.1111/j.1399-0039.1980.tb00285.x. View

20.

Nadler L, Stashenko P, Hardy R, Tomaselli K, Yunis E, Schlossman S . Monoclonal antibody identifies a new Ia-like (p29,34) polymorphic system linked to the HLA-D/DR region. Nature. 1981; 290(5807):591-3. DOI: 10.1038/290591a0. View