Rapid Enzyme-linked Immunosorbent Assay (ELISA) for Aspergillus Fumigatus Antibodies

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1982 Oct 1

PMID 6813358

Citations 9

Authors

M D Richardson

J M Stubbins

D W Warnock

Affiliations

Soon will be listed here.

Abstract

A rapid enzyme-linked immunosorbent assay (ELISA) where component incubation periods were shortened to one hour, was compared with agar gel double diffusion (AGDD) and a standard ELISA procedure for detecting antibodies to Aspergillus fumigatus in 28 asthmatic patients with suspected allergic aspergillosis. Using two A fumigatus antigens the rapid ELISA compared well with AGDD and the standard ELISA method. Eleven sera that reacted with both antigens in AGDD were all positive against antigen 1 in both forms of ELISA, but two failed to react with antigen 2 in the standard ELISA and three failed to react with this antigen in the rapid method. Thirteen AGDD-negative sera were also negative in both forms of ELISA. The rapid ELISA provides a sensitive and reproducible test for routine serological investigation of allergic aspergillosis.

Citing Articles

Evaluation of the LDBio Aspergillus ICT lateral flow assay for serodiagnosis of allergic bronchopulmonary aspergillosis.

Hunter E, Page I, Richardson M, Denning D PLoS One. 2020; 15(9):e0238855.

PMID: 32976540 PMC: 7518618. DOI: 10.1371/journal.pone.0238855.

Chronic Pulmonary Aspergillosis: Notes for a Clinician in a Resource-Limited Setting Where There Is No Mycologist.

Bongomin F, Asio L, Baluku J, Kwizera R, Denning D J Fungi (Basel). 2020; 6(2).

PMID: 32498415 PMC: 7345130. DOI: 10.3390/jof6020075.

Evaluation of LDBio ICT Lateral Flow Assay for IgG and IgM Antibody Detection in Chronic Pulmonary Aspergillosis.

Hunter E, Richardson M, Denning D J Clin Microbiol. 2019; 57(9).

PMID: 31217272 PMC: 6711894. DOI: 10.1128/JCM.00538-19.

Evaluation of the Double Agar Gel Immunodiffusion Test and of the Enzyme-Linked Immunosorbent Assay in the Diagnosis and Follow-Up of Patients with Chronic Pulmonary Aspergillosis.

de Azevedo P, Sylvestre T, Cavalcante R, de Carvalho L, Moris D, Oliveira M PLoS One. 2015; 10(8):e0134841.

PMID: 26271000 PMC: 4536220. DOI: 10.1371/journal.pone.0134841.

Enilconazole as an adjunct to the treatment of four cases of canine nasal aspergillosis.

Lanthier T, Chalifoux A Can Vet J. 1991; 32(2):110-2.

PMID: 17423733 PMC: 1480943.

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