Radioimmunoassay for Detection of Antibodies to Neisseria Gonorrhoeae

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1982 Jun 1

PMID 6809784

Authors

M Usategui

E V Savard

S M Mondabaugh

N L Keigher

Affiliations

Soon will be listed here.

Abstract

A radioimmunoassay has been developed and evaluated for the serological diagnosis of gonorrhea. Purified gonococcal antigen was obtained from a culture of Neisseria gonorrhoeae (B370) and labeled with 125I for use in a double-antibody test system. The test was evaluated in populations segregated by sex and risk. The specificity of the assay in females was 90.2% (55/61) in low risk, 82.2% (2,245/ 2,732) in medium risk, and 54.1% (335/619) in high risk. The sensitivity was 69% (20/29) in medium risk and 78.3% (288/367) in high risk. In males, test specificity was 92.3% (24/26) in low risk and 50% (48/96) in high risk. The sensitivity was 70.8% (143/202) in the high-risk group. The data in this study indicate that this assay should not be employed for screening of either high- or medium-risk populations.

References

Caloenescu M, CLECNER B, Petrow S, Kasatiya S . Immunofluorescent antibody test for diagnosis of gonorrhoea. J Clin Microbiol. 1975; 1(2):143-6. PMC: 274989. DOI: 10.1128/jcm.1.2.143-146.1975. View

Pariser D, Pittard 3rd W . Primary isolation of Neisseria gonorrhoeae on hemoglobin-free Thayer-Martin medium. Public Health Rep (1896). 1970; 85(6):532-4. PMC: 2031733. View

Koransky J, Jacobs Jr N . Serologic testing for gonorrhea. Sex Transm Dis. 1977; 4(1):27-31. DOI: 10.1097/00007435-197701000-00009. View

Hallqvist L, Lindgren S . Gonorrhoea of the throat at a venereological clinic. Incidence and Results of Treatment. Br J Vener Dis. 1975; 51(6):395-7. PMC: 1045189. DOI: 10.1136/sti.51.6.395. View

Handsfield H, Lipman T, Harnisch J, Tronca E, Holmes K . Asymptomatic gonorrhea in men. Diagnosis, natural course, prevalence and significance. N Engl J Med. 1974; 290(3):117-23. DOI: 10.1056/NEJM197401172900301. View

REYN A, Bentzon M . Comparison of a selective and a non-selective medium in the diagnosis of gonorrhoea to ascertain the sensitivity of Neisseria gonorrhoeae to vancomycin. Br J Vener Dis. 1972; 48(5):363-8. PMC: 1048343. DOI: 10.1136/sti.48.5.363. View

Chipperfield E, Catterall R . Reappraisal of Gram-staining and cultural techniques for the diagnosis of gonorrhoea in women. Br J Vener Dis. 1976; 52(1):36-9. PMC: 1045208. DOI: 10.1136/sti.52.1.36. View

TOSHACH S, Coull I, Sigurdson S, Dublenko S, Grocholski J, Linarez L . Evaluation of five serologic tests for antibody to Neisseria gonorrhoeae. Sex Transm Dis. 1979; 6(3):214-7. DOI: 10.1097/00007435-197907000-00006. View

Gnarpe H, Wallin J . Studies in venereal disease. II. Improved diagnosis of gonorrhoea by the parallel use of conventional and L-phase media for culture. Br J Vener Dis. 1973; 49(6):505-7. PMC: 1048394. DOI: 10.1136/sti.49.6.505. View

10.

Faur Y, Weisburd M, Wilson M . A new medium for the isolation of pathogenic Neisseria (NYC nmedium). 3. Performance as a culture and transport medium without addition of ambient carbon dioxide. Health Lab Sci. 1973; 10(2):61-74. View

11.

Gaafar H . Serologic diagnosis of gonococcal infection. Arch Androl. 1979; 3(4):337-41. DOI: 10.3109/01485017908988425. View

12.

Crawford C, Knapp J, Hale J, Holmes K . Asymptomatic gonorrhea in men: caused by gonococci with unique nutritional requirements. Science. 1977; 196(4296):1352-3. DOI: 10.1126/science.405742. View

13.

Murphy E, Abbey H . The normal range--a common misuse. J Chronic Dis. 1967; 20(2):79-88. DOI: 10.1016/0021-9681(67)90099-9. View

14.

Hunter W, GREENWOOD F . Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962; 194:495-6. DOI: 10.1038/194495a0. View

15.

Rothenberg R, Simon R, Chipperfield E, Catterall R . Efficacy of selected diagnostic tests for sexually transmitted diseases. JAMA. 1976; 235(1):49-51. View

16.

Norins L . The case for gonococcal serology. J Infect Dis. 1974; 130(6):677-9. DOI: 10.1093/infdis/130.6.677. View

17.

Gaafar H, DArcangelis D . Fluorescent antibody test for the serological diagnosis of gonorrhea. J Clin Microbiol. 1976; 3(4):438-42. PMC: 274320. DOI: 10.1128/jcm.3.4.438-442.1976. View

18.

Brorson J, Holmberg I, Nygren B, Seeberg S . Vancomycin-sensitive strains of Neisseria gonorrhoeae. A problem for the diagnostic laboratory. Br J Vener Dis. 1973; 49(5):452-3. PMC: 1044955. DOI: 10.1136/sti.49.5.452. View

19.

Symington D . Improved transport system for Neisseria gonorrhoeae in clinical specimens. J Clin Microbiol. 1975; 2(6):498-503. PMC: 275210. DOI: 10.1128/jcm.2.6.498-503.1975. View

20.

Chapel T, Smeltzer M . Culture of urinary sediment for the diagnosis of gonorrhoea in women. Br J Vener Dis. 1975; 51(1):25-7. PMC: 1045103. DOI: 10.1136/sti.51.1.25. View