Evidence for a Role of Calmodulin in Serum Stimulation of Na+ Influx in Human Fibroblasts

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Jun 1

PMID 6808512

Citations 18

Authors

N E Owen

M L Villereal

Affiliations

Soon will be listed here.

Abstract

Sodium influx in serum-deprived human diploid fibroblasts can be stimulated by addition of serum (5-fold) or the divalent cation ionophore A23187 (3-fold). The possible involvement of calmodulin in serum or A23187 stimulation of Na+ influx has been investigated by using six psychoactive agents that are known to bind calmodulin and inhibit calmodulin-sensitive enzymes. Each agent inhibited serum- and A23187-stimulated Na+ influx in a dose-dependent manner. Furthermore, the K1 for inhibition of serum-stimulated Na+ influx correlates directly with the Ca2+-specific calmodulin binding previously determined in a cell-free system [Levin, R. M. & Weiss, B. (1979) J. Pharmacol. Exp, Ther. 208, 454--459]. None of the agents tested had any effect on the serum-insensitive component of net Na+ influx in these cells. These data support the concept that serum and A23187 stimulate Na+ influx in human diploid fibroblasts via an increase of intracellular Ca2+ and a subsequent calmodulin-mediated activation of the amiloride-sensitive transport pathway.

Citing Articles

Effect of Ca and Calmodulin on DeltapH Formation in Tonoplast Vesicles from Corn Roots.

Ladror U, Zielinski R Plant Physiol. 1990; 92(3):850-4.

PMID: 16667359 PMC: 1062379. DOI: 10.1104/pp.92.3.850.

Intracellular calcium and calmodulin involvement in protoplast fusion.

Grimes H, Boss W Plant Physiol. 1985; 79(1):253-8.

PMID: 16664381 PMC: 1074861. DOI: 10.1104/pp.79.1.253.

Intracellular calcium mobilization and activation of the Na+/H+ exchanger in platelets.

Poch E, Botey A, Gaya J, Cases A, Rivera F, Revert L Biochem J. 1993; 290 ( Pt 2):617-22.

PMID: 8383969 PMC: 1132319. DOI: 10.1042/bj2900617.

Ion content and the response of L5178Y-R and L5178Y-S cells to X rays and ionophore A23187.

Szumiel I, Wlodek D, Lustyik G Radiat Environ Biophys. 1984; 23(4):255-67.

PMID: 6436901 DOI: 10.1007/BF01407597.

Trifluoperazine, a calmodulin antagonist, inhibits muscle cell fusion.

Bar-Sagi D, Prives J J Cell Biol. 1983; 97(5 Pt 1):1375-80.

PMID: 6415064 PMC: 2112681. DOI: 10.1083/jcb.97.5.1375.

References

Weber M, Rubin H . Uridine transport and RNA synthesis in growing and in density-inhibited animal cells. J Cell Physiol. 1971; 77(2):157-68. DOI: 10.1002/jcp.1040770205. View

Sefton B, Rubin H . Stimulation of glucose transport in cultures of density-inhibited chick embryo cells. Proc Natl Acad Sci U S A. 1971; 68(12):3154-7. PMC: 389611. DOI: 10.1073/pnas.68.12.3154. View

Reed P, LARDY H . A23187: a divalent cation ionophore. J Biol Chem. 1972; 247(21):6970-7. View

Seeman P . The membrane actions of anesthetics and tranquilizers. Pharmacol Rev. 1972; 24(4):583-655. View

Malagodi M, Chiou C . Pharmacological evaluation of a new Ca2+ antagonist, 8-(N,N-diethylamino)-octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8): studies in smooth muscles. Eur J Pharmacol. 1974; 27(1):25-33. DOI: 10.1016/0014-2999(74)90198-8. View

Uzunov P, SHEIN H, Weiss B . Multiple forms of cyclic 3',5'-AMP phosphodiesterase of rat cerebrum and cloned astrocytoma and neuroblastoma cells. Neuropharmacology. 1974; 13(5):377-91. DOI: 10.1016/0028-3908(74)90120-8. View

Cuthbert A, Wong P . Calcium release in relation to permeability changes in toad bladder epithelium following antidiuretic hormone. J Physiol. 1974; 241(2):407-22. PMC: 1331039. DOI: 10.1113/jphysiol.1974.sp010663. View

Rozengurt E, HEPPEL L . Serum rapidly stimulates ouabain-sensitive 86-RB+ influx in quiescent 3T3 cells. Proc Natl Acad Sci U S A. 1975; 72(11):4492-5. PMC: 388748. DOI: 10.1073/pnas.72.11.4492. View

Le Breton G, Sandler W, FEINBERG H . The effect of D2O and chlortetracycline on ADP-induced platelet shape change and aggregation. Thromb Res. 1976; 8(4):477-85. DOI: 10.1016/0049-3848(76)90225-5. View

10.

Johnson J, Epel D . Intracellular pH and activation of sea urchin eggs after fertilisation. Nature. 1976; 262(5570):661-4. DOI: 10.1038/262661a0. View

11.

Tupper J, Del Rosso M, Hazelton B, Zorgniotti F . Serum-stimulated changes in calcium transport and distribution in mouse 3T3 cells and their modification by dibutyryl cyclic AMP. J Cell Physiol. 1978; 95(1):71-84. DOI: 10.1002/jcp.1040950110. View

12.

Schulman H, Greengard P . Ca2+-dependent protein phosphorylation system in membranes from various tissues, and its activation by "calcium-dependent regulator". Proc Natl Acad Sci U S A. 1978; 75(11):5432-6. PMC: 392978. DOI: 10.1073/pnas.75.11.5432. View

13.

Smith J, Rozengurt E . Serum stimulates the Na+,K+ pump in quiescent fibroblasts by increasing Na+ entry. Proc Natl Acad Sci U S A. 1978; 75(11):5560-4. PMC: 393006. DOI: 10.1073/pnas.75.11.5560. View

14.

Levin R, Weiss B . Selective binding of antipsychotics and other psychoactive agents to the calcium-dependent activator of cyclic nucleotide phosphodiesterase. J Pharmacol Exp Ther. 1979; 208(3):454-9. View

15.

Taub M, Saier Jr M . Regulation of 22Na+ transport by calcium in an established kidney epithelial cell line. J Biol Chem. 1979; 254(22):11440-4. View

16.

Koch K, Leffert H . Increased sodium ion influx is necessary to initiate rat hepatocyte proliferation. Cell. 1979; 18(1):153-63. DOI: 10.1016/0092-8674(79)90364-7. View

17.

Cheung W . Calmodulin plays a pivotal role in cellular regulation. Science. 1980; 207(4426):19-27. DOI: 10.1126/science.6243188. View

18.

Levin R, Weiss B . Inhibition by trifluoperazine of calmodulin-induced activation of ATPase activity of rat erythrocyte. Neuropharmacology. 1980; 19(2):169-74. DOI: 10.1016/0028-3908(80)90134-3. View

19.

Means A, Dedman J . Calmodulin--an intracellular calcium receptor. Nature. 1980; 285(5760):73-7. DOI: 10.1038/285073a0. View

20.

Klee C, CROUCH T, Richman P . Calmodulin. Annu Rev Biochem. 1980; 49:489-515. DOI: 10.1146/annurev.bi.49.070180.002421. View