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Identification of the 48-base-long Primordial Building Block Sequence of Mouse Immunoglobulin Variable Region Genes

Overview
Journal Proc Natl Acad Sci U S A
Specialty Science
Date 1982 Mar 1
PMID 6804949
Citations 10
Authors
S Ohno
T Matsunaga
R B Wallace
Affiliations
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Abstract

Mouse immunoglobulin heavy-chain variable region (Ig VH) genes apparently arose from the approximately 600-base-pair-long (approximately 12 tandem repeats of the 48-base-pair-long primordial building block sequence TTC-AGC-AGC-CTG-ACT-GGA-TAT-GAC-CTG-GAG-TGG-ACT-TAC-TGC-GCA-AGA) that in the original reading frame specified the amino acid sequence Phe-Ser-Ser-Leu-Thr-Gly-Tyr-Asp-Leu-Glu-Trp-Thr-Tyr-Cys-Ala-Arg. The previously identified, shorter prototype building blocks merely represented particular portions of the above primordial sequence. Even today, the direct descendant in toto of this primordial sequence specifies the last one-sixth of each VH coding sequence: the 83rd to 98th amino acid residues. Furthermore, its four truncated derivatives specify the 4th to 14th, 17th to 23rd, 29th to 37th, and 38th to 48th amino acid residues. Accordingly, all three relatively invariant--therefore, conserved--framework regions (FW-1, FW-2, and FW-3) of VHs are specified by recognizable--therefore, conserved--descendants of the primordial sequence.

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Sequence similarities of protein kinase peptide substrates and inhibitors: comparison of their primary structures with immunoglobulin repeats.

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The genome of the THE I human transposable repetitive elements is composed of a basic motif homologous to an ancestral immunoglobulin gene sequence.

Hakim I, Amariglio N, Grossman Z, Ohno S, Rechavi G Proc Natl Acad Sci U S A. 1994; 91(17):7967-9.

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Identification of the 45-base-long primordial building block of the entire class I major histocompatibility complex antigen gene.

Ohno S, Matsunaga T, Epplen J, Itakura K, Wallace R Proc Natl Acad Sci U S A. 1982; 79(20):6342-6.

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The primitive code and repeats of base oligomers as the primordial protein-encoding sequence.

Ohno S, Epplen J Proc Natl Acad Sci U S A. 1983; 80(11):3391-5.

PMID: 6574491 PMC: 394049. DOI: 10.1073/pnas.80.11.3391.

References
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