Isolation and Characterization of Circulating Immune Complexes in Cystic Fibrosis

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1982 Feb 1

PMID 6804143

Citations 6

Authors

R B Moss

Y P Hsu

Affiliations

Soon will be listed here.

Abstract

A methodology for the isolation and immunologic characterization of IgG-containing circulating immune complexes (IgG-CIC) as detected by the 125IClq-binding assay (ClqBA) is described. We applied this methodology to sera from patients with cystic fibrosis (CF), both positive and negative for IgG-CIC. We used latex-fixation-positive rheumatoid arthritis sera and normal human sera as positive and negative controls, respectively. All IgG-CIC-positive serum samples from CF patients were found to contain antibodies against Pseudomonas aeruginosa in the isolated complexes. Some patients also had antibodies in CIC specific for Staphylococcus aureus and Candida albicans. CIC specificity corresponded to respiratory tract colonization for each patient.

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References

Gray B . ELISA methodology for polysaccharide antigens: protein coupling of polysaccharides for adsorption to plastic tubes. J Immunol Methods. 1979; 28(1-2):187-92. DOI: 10.1016/0022-1759(79)90340-5. View

Harkiss G, Brown D, Evans D . Longitudinal study of circulating immune complexes in a patient with Staphylococcus albus-induced shunt nephritis. Clin Exp Immunol. 1979; 37(2):228-38. PMC: 1537820. View

Casali P, Lambert P . Purification of soluble immune complexes from serum using polymethylmetacrylate beads coated with conglutinin or C1q. Application to the analysis of the components of in vitro formed immune complexes and of immune complexes occurring in vivo during.... Clin Exp Immunol. 1979; 37(2):295-309. PMC: 1537812. View

Pernice W, Sodomann C, Luben G, Seiler F, Sedlacek H . Antigen-specific detection of HBsAG-containing immune complexes in the course of hepatitis B virus infection. Clin Exp Immunol. 1979; 37(2):376-80. PMC: 1537802. View

Moss R, Lewiston N . Immune complexes and humoral response to Pseudomonas aeruginosa in cystic fibrosis. Am Rev Respir Dis. 1980; 121(1):23-9. DOI: 10.1164/arrd.1980.121.1.23. View

Theofilopoulos A, Dixon F . The biology and detection of immune complexes. Adv Immunol. 1979; 28:89-220. DOI: 10.1016/s0065-2776(08)60800-7. View

Berdischewsky M, Pollack M, Young L, Chia D, Osher A, Barnett E . Circulating immune complexes in cystic fibrosis. Pediatr Res. 1980; 14(6):830-3. DOI: 10.1203/00006450-198006000-00011. View

Kilpatrick J, Virella G . Isolation and characterization of soluble insulin-anti-insulin immune complexes formed in vitro and in vivo in sera from patients with diabetes mellitus. Clin Exp Immunol. 1980; 40(3):445-52. PMC: 1538931. View

Yoshinoya S, Cox R, Pope R . Circulating immune complexes in coccidioidomycosis. Detection and characterization. J Clin Invest. 1980; 66(4):655-63. PMC: 371638. DOI: 10.1172/JCI109901. View

10.

Tabbarah Z, Wheat L, Kohler R, White A . Thermodissociation of staphylococcal immune complexes and detection of staphylococcal antigen in serum from patients with Staphylococcus aureus bacteremia. J Clin Microbiol. 1980; 11(6):703-9. PMC: 273490. DOI: 10.1128/jcm.11.6.703-709.1980. View

11.

Denaro M, Romeo G . Antibodies cross-reacting with porcine and human trypsin in cystic fibrosis. Pediatr Res. 1980; 14(9):1086-7. DOI: 10.1203/00006450-198009000-00014. View

12.

Moss R, Hsu Y, Lewiston N . 125I-Clq-binding and specific antibodies as indicators of pulmonary disease activity in cystic fibrosis. J Pediatr. 1981; 99(2):215-22. DOI: 10.1016/s0022-3476(81)80453-2. View

13.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

14.

GREENWOOD F, Hunter W, GLOVER J . THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY. Biochem J. 1963; 89:114-23. PMC: 1202279. DOI: 10.1042/bj0890114. View

15.

Stein A, MANLAPAS F, SOIKE K, PATTERSON P . SPECIFIC ISOANTIBODIES IN CYSTIC FIBROSIS. A STUDY OF SERUM AND BRONCHIAL MUCUS. J Pediatr. 1964; 65:495-500. DOI: 10.1016/s0022-3476(64)80285-7. View

16.

Hanessian S, Regan W, WATSON D, HASKELL T . Isolation and characterization of antigenic components of a new heptavalent Pseudomonas vaccine. Nat New Biol. 1971; 229(7):209-10. DOI: 10.1038/newbio229209a0. View

17.

McFarlane H, Holzel A, Brenchley P, Allan J, Wallwork J, Singer B . Immune complexes in cystic fibrosis. Br Med J. 1975; 1(5955):423-8. PMC: 1672436. DOI: 10.1136/bmj.1.5955.423. View

18.

Hoiby N, Wiik A . Antibacterial precipitins and autoantibodies in serum of patients with cystic fibrosis. Scand J Respir Dis. 1975; 56(1):38-46. View

19.

Wallwork J, Brenchley P, McCarthy J, Allan J, Moss D, Ward A . Some aspects of immunity in patients with cystic fibrosis. Clin Exp Immunol. 1974; 18(3):303-20. PMC: 1537996. View

20.

Wood R, Boat T, DOERSHUK C . Cystic fibrosis. Am Rev Respir Dis. 1976; 113(6):833-78. DOI: 10.1164/arrd.1976.113.6.833. View