Human Intestinal Potential Difference: Recording Method and Biophysical Implications

Overview

Journal J Physiol

Specialty Physiology

Date 1981 Dec 1

PMID 6802960

Citations 7

Authors

R F Gustke

P McCormick

H Ruppin

K H SOERGEL

G E Whalen

C M Wood

Affiliations

Soon will be listed here.

Abstract

1. The transmural electrical potential difference (PD) of the intact human small intestine was recorded with close attention to electrical symmetry, shielding from electro-magnetic waves and correction for junction potentials. 2. The PD is -12 mV (mucosa-negative) in the fasting jejunum and ileum and does not change during perfusion with isotonic NaCl. 3. Absorption of Na and Cl appears to be non-electrogenic and the 'resting' PD is probably generated by active anion secretion of fasting intestinal contents. 4. Diffusion potentials during isotonic D-mannitol perfusion indicated higher cation selectivity in the ileum than in the jejunum. 5. The calculated contribution of a free-solution path to total paracellular permeability is 55% in the jejunum but only 15% in the ileum. 6. No 'streaming' potential was detected during osmotic water flow, suggesting that the cation-selectivity of the channels is temporarily inactivated during dilatation of the lateral intercellular space.

Citing Articles

Bioelectric medicine: unveiling the therapeutic potential of micro-current stimulation.

Lee H, Cho S, Kim D, Lee T, Kim H Biomed Eng Lett. 2024; 14(3):367-392.

PMID: 38645592 PMC: 11026362. DOI: 10.1007/s13534-024-00366-3.

Minimally Invasive Image-Guided Gut Transport Function Measurement Probe.

Otuya D, Gavgiotaki E, Carlson C, Shi S, Lee A, Krall A Front Phys. 2022; 9.

PMID: 36382063 PMC: 9648666. DOI: 10.3389/fphy.2021.735645.

Passively scanned, single-fiber optical coherence tomography probes for gastrointestinal devices.

Otuya D, Dechene N, Poshtupaka D, Judson S, Carlson C, Zemlok S Lasers Surg Med. 2022; 54(7):935-944.

PMID: 35708124 PMC: 9541095. DOI: 10.1002/lsm.23576.

Developmental Changes in Phosphate Homeostasis.

MacDonald T, Saurette M, Beggs M, Alexander R Rev Physiol Biochem Pharmacol. 2021; 179:117-138.

PMID: 33398502 DOI: 10.1007/112_2020_52.

Infection-generated electric field in gut epithelium drives bidirectional migration of macrophages.

Sun Y, Reid B, Ferreira F, Luxardi G, Ma L, Lokken K PLoS Biol. 2019; 17(4):e3000044.

PMID: 30964858 PMC: 6456179. DOI: 10.1371/journal.pbio.3000044.

References

SMYTH D, Wright E . Streaming potentials in the rat small intestine. J Physiol. 1966; 182(3):591-602. PMC: 1357489. DOI: 10.1113/jphysiol.1966.sp007839. View

HODGKIN A, Katz B . The effect of sodium ions on the electrical activity of giant axon of the squid. J Physiol. 1949; 108(1):37-77. PMC: 1392331. DOI: 10.1113/jphysiol.1949.sp004310. View

Wright E . Diffusion potentials across the small intestine. Nature. 1966; 212(5058):189-90. DOI: 10.1038/212189a0. View

GRADY G, Madoff M, Duhamel R, Moore E, CHALMERS T . Sodium transport by human ileum in vitro and its response to cholera enterotoxin. Gastroenterology. 1967; 53(5):737-44. View

SOERGEL K, Whalen G, Harris J . Passive movement of water and sodium across the human small intestinal mucosa. J Appl Physiol. 1968; 24(1):40-8. DOI: 10.1152/jappl.1968.24.1.40. View

Levin R, KOLDOVSKY O, Hoskova J, JIRSOVA V, Uher J . Electrical activity across human foetal small intestine associated with absorption processes. Gut. 1968; 9(2):206-13. PMC: 1552566. DOI: 10.1136/gut.9.2.206. View

Sachar D, Taylor J, Saha J, Phillips R . Intestinal transmural electric potential and its response to glucose in acute and convalescent cholera. Gastroenterology. 1969; 56(3):512-21. View

HERNANDEZ N, Beck I . Gastroesophageal transmural potential difference measured by a new constant infusion method. The effect of skin scarification on this potential difference. Am J Dig Dis. 1969; 14(3):206-16. DOI: 10.1007/BF02235884. View

Turnberg L, Fordtran J, Carter N, RECTOR Jr F . Mechanism of bicarbonate absorption and its relationship to sodium transport in the human jejunum. J Clin Invest. 1970; 49(3):548-56. PMC: 322503. DOI: 10.1172/JCI106265. View

10.

Turnberg L, Bieberdorf F, Morawski S, Fordtran J . Interrelationships of chloride, bicarbonate, sodium, and hydrogen transport in the human ileum. J Clin Invest. 1970; 49(3):557-67. PMC: 322504. DOI: 10.1172/JCI106266. View

11.

Grantham R, Code C, SCHLEGEL J . Reference electrode sites in determination of potential difference across the gastroesophageal mucosal junction. Mayo Clin Proc. 1970; 45(4):265-74. View

12.

Way L, Diamond J . The effect of secretin on electrical potential differences in the pancreatic duct. Biochim Biophys Acta. 1970; 203(2):298-307. DOI: 10.1016/0005-2736(70)90144-6. View

13.

Frizzell R, Schultz S . Ionic conductances of extracellular shunt pathway in rabbit ileum. Influence of shunt on transmural sodium transport and electrical potential differences. J Gen Physiol. 1972; 59(3):318-46. PMC: 2203181. DOI: 10.1085/jgp.59.3.318. View

14.

Beck I, Dinda P . On the mechanism of isoosmotic transport of fluid across the small intestine. The effect of the Staverman reflection coefficient of the solute used to increase the osmolality of the mucosal solution on the composition of the absorbate. Can J Physiol Pharmacol. 1974; 52(1):96-104. DOI: 10.1139/y74-013. View

15.

Moreno J, Diamond J . Discrimination of monovalent inorganic cations by "tight" junctions of gallbladder epithelium. J Membr Biol. 1974; 15(3):277-318. DOI: 10.1007/BF01870092. View

16.

Wade J, Karnovsky M . Fracture faces of osmotically disrupted zonulae occludentes. J Cell Biol. 1974; 62(2):344-50. PMC: 2109392. DOI: 10.1083/jcb.62.2.344. View

17.

Mandel L . Actions of external hypertonic urea, ADH, and theophylline on transcellular and extracellular solute permeabilities in frog skin. J Gen Physiol. 1975; 65(5):599-615. PMC: 2214885. DOI: 10.1085/jgp.65.5.599. View

18.

Isaacs P, Horth C, Turnberg L . The electrical potential difference across human ileostomy mucosa. Gastroenterology. 1976; 70(1):52-8. View

19.

Frizzell R, Koch M, Schultz S . Ion transport by rabbit colon. I. Active and passive components. J Membr Biol. 1976; 27(3):297-316. DOI: 10.1007/BF01869142. View

20.

Corbett C, Isaacs P, Riley A, Turnberg L . Human intestinal ion transport in vitro. Gut. 1977; 18(2):136-40. PMC: 1411298. DOI: 10.1136/gut.18.2.136. View