Sheep, Rabbit and Chicken Antisera Against a Human VH Fragment: Reactivity with Immunoglobulins and Lymphocytes

Overview

Journal Immunology

Specialty Allergy & Immunology

Date 1982 Apr 1

PMID 6802746

Citations 4

Authors

T E Michaelsen

T Lea

Affiliations

Soon will be listed here.

Abstract

Antisera against a human VH fragment obtained from an IgG3, VH II, kappa protein (KUP) were raised in rabbits, sheep and chicken. The three types of anti-VH antisera reacted equally well with both intact immunoglobulin molecules and isolated heavy chains. The antisera did not detect any free heavy chain specific antigens by sensitive enzyme-linked immunosorbent assay (ELISA) tests although they reacted with some antigens which were more or less hidden on intact immunoglobulin molecules but well expressed on isolated heavy chains. The antisera reacted with more than 90% of IgG, IgA and IgM present in normal pooled serum. Experiments with T cells from normal peripheral blood indicated that the sheep and rabbit anti-VH antisera reacted with a 70,000 mol.wt T-cell surface antigen.

Citing Articles

Anti-VH antibodies interfere with antigen binding by human T lymphocytes.

Lea T, Michaelsen T Clin Exp Immunol. 1982; 49(3):657-64.

PMID: 6216996 PMC: 1536729.

Expression of immunoglobulin-cross-reactive molecules by neoplastic human T cells. I. Surface detection and isolation of molecules reactive with chicken anti-F(ab')2, anti-alpha and anti-mu antibodies.

Haegert D, Timm M Immunology. 1983; 50(3):447-59.

PMID: 6195097 PMC: 1454261.

Demonstration of non-idiotypic variable heavy chain (VH) antigens on human peripheral blood lymphocytes.

Lea T, Michaelsen T, Natvig J Immunology. 1982; 46(4):849-57.

PMID: 6179859 PMC: 1555468.

IgG subclass distribution of anti-Rh, anti-Kell and anti-Duffy antibodies measured by sensitive haemagglutination assays.

Michaelsen T, Kornstad L Clin Exp Immunol. 1987; 67(3):637-45.

PMID: 3111763 PMC: 1542629.

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