Distinct Galactose Phosphoenolpyruvate-dependent Phosphotransferase System in Streptococcus Lactis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Feb 1

PMID 6799488

Citations 12

Authors

Y H Park

L L McKay

Affiliations

Soon will be listed here.

Abstract

Lactose-negative (Lac-) mutants were isolated from a variant of Streptococcus lactis C2 in which the lactose plasmid had become integrated into the chromosome. These mutants retained their parental growth characteristics on galactose (Lac- Gal+). This is in contrast to the Lac- variants obtained when the lactose plasmid is lost from S. lactis, which results in a slower growth rate on galactose (Lac- Gal+). The Lac- Gal+ mutants were defective in [14C]thiomethyl-beta-D-galactopyranoside accumulation, suggesting a defect in the lactose phosphoenolpyruvate-dependent phosphotransferase system, but still possessed the ability to form galactose-1-phosphate and galactose-6-phosphate from galactose in a ratio similar to that observed from the parental strain. The Lac- Gald variant formed only galactose-1-phosphate. The results imply that galactose is not translocated via the lactose phosphoenolpyruvate-dependent phosphotransferase system, but rather by a specific galactose phosphoenolpyruvate-dependent phosphotransferase system for which the genetic locus is also found on the lactose plasmid in S. lactis.

Citing Articles

Further Elucidation of Galactose Utilization in MG1363.

Solopova A, Bachmann H, Teusink B, Kok J, Kuipers O Front Microbiol. 2018; 9:1803.

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Towards enhanced galactose utilization by Lactococcus lactis.

Neves A, Pool W, Solopova A, Kok J, Santos H, Kuipers O Appl Environ Microbiol. 2010; 76(21):7048-60.

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Galactose Expulsion during Lactose Metabolism in Lactococcus lactis subsp. cremoris FD1 Due to Dephosphorylation of Intracellular Galactose 6-Phosphate.

Benthin S, Nielsen J, Villadsen J Appl Environ Microbiol. 1994; 60(4):1254-9.

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Properties of Lactose Plasmid pLY101 in Lactobacillus casei.

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Characterization, expression, and mutation of the Lactococcus lactis galPMKTE genes, involved in galactose utilization via the Leloir pathway.

Grossiord B, Luesink E, Vaughan E, Arnaud A, de Vos W J Bacteriol. 2003; 185(3):870-8.

PMID: 12533462 PMC: 142802. DOI: 10.1128/JB.185.3.870-878.2003.

References

Langridge J . Mutations conferring quantitative and qualitative increases in beta-galactosidase activity in Escherichia coli. Mol Gen Genet. 1969; 105(1):74-83. DOI: 10.1007/BF00750315. View

McKay L, Baldwin K . Stabilization of Lactose Metabolism in Streptococcus lactis C2. Appl Environ Microbiol. 1978; 36(2):360-7. PMC: 291226. DOI: 10.1128/aem.36.2.360-367.1978. View

Klaenhammer T, McKay L, Baldwin K . Improved lysis of group N streptococci for isolation and rapid characterization of plasmid deoxyribonucleic acid. Appl Environ Microbiol. 1978; 35(3):592-600. PMC: 242884. DOI: 10.1128/aem.35.3.592-600.1978. View

McKay L, Miller 3rd A, Sandine W, Elliker P . Mechanisms of lactose utilization by lactic acid streptococci: enzymatic and genetic analyses. J Bacteriol. 1970; 102(3):804-9. PMC: 247630. DOI: 10.1128/jb.102.3.804-809.1970. View

Thompson J . Galactose transport systems in Streptococcus lactis. J Bacteriol. 1980; 144(2):683-91. PMC: 294718. DOI: 10.1128/jb.144.2.683-691.1980. View

Leblanc D, Crow V, Lee L, Garon C . Influence of the lactose plasmid on the metabolism of galactose by Streptococcus lactis. J Bacteriol. 1979; 137(2):878-84. PMC: 218370. DOI: 10.1128/jb.137.2.878-884.1979. View

Bissett D, Anderson R . Lactose and D-galactose metabolism in group N streptococci: presence of enzymes for both the D-galactose 1-phosphate and D-tagatose 6-phosphate pathways. J Bacteriol. 1974; 117(1):318-20. PMC: 246560. DOI: 10.1128/jb.117.1.318-320.1974. View

Thompson J, Turner K, Thomas T . Catabolite inhibition and sequential metabolism of sugars by Streptococcus lactis. J Bacteriol. 1978; 133(3):1163-74. PMC: 222148. DOI: 10.1128/jb.133.3.1163-1174.1978. View

McKay L, Baldwin K, Efstathiou J . Transductional evidence for plasmid linkage of lactose metabolism in streptococcus lactis C2. Appl Environ Microbiol. 1976; 32(1):45-52. PMC: 170003. DOI: 10.1128/aem.32.1.45-52.1976. View

10.

Thomas T, Jarvis B, Skipper N . Localization of proteinase(s) near the cell surface of Streptococcus lactis. J Bacteriol. 1974; 118(2):329-33. PMC: 246762. DOI: 10.1128/jb.118.2.329-333.1974. View

11.

Cords B, McKay L . Characterization of lactose-fermenting revertants from lactose-negative Streptococcus lactis C2 mutants. J Bacteriol. 1974; 119(3):830-9. PMC: 245687. DOI: 10.1128/jb.119.3.830-839.1974. View

12.

McKay L, Baldwin K . Induction of prophage in Streptococcus lactis C2 by ultraviolet irradiation. Appl Microbiol. 1973; 25(4):682-4. PMC: 380883. DOI: 10.1128/am.25.4.682-684.1973. View

13.

Hengstenberg W, Penberthy W, Hill K, Morse M . Metabolism of lactose by Staphylococcus aureus. J Bacteriol. 1968; 96(6):2187-8. PMC: 252586. DOI: 10.1128/jb.96.6.2187-2188.1968. View

14.

McKay L, Walter L, Sandine W, Elliker P . Involvement of phosphoenolpyruvate in lactose utilization by group N streptococci. J Bacteriol. 1969; 99(2):603-10. PMC: 250061. DOI: 10.1128/jb.99.2.603-610.1969. View

15.

Harvey R, Collins E . ROLES OF CITRATE AND ACETOIN IN THE METABOLISM OF STREPTOCOCCUS DIACETILACTIS. J Bacteriol. 1963; 86:1301-7. PMC: 283645. DOI: 10.1128/jb.86.6.1301-1307.1963. View

16.

Morse M, Hill K, Egan J, Hengstenberg W . Metabolism of lactose by Staphylococcus aureus and its genetic basis. J Bacteriol. 1968; 95(6):2270-4. PMC: 315162. DOI: 10.1128/jb.95.6.2270-2274.1968. View

17.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

18.

McKay L, Baldwin K, Zottola E . Loss of lactose metabolism in lactic streptococci. Appl Microbiol. 1972; 23(6):1090-6. PMC: 380512. DOI: 10.1128/am.23.6.1090-1096.1972. View

19.

Thompson J, Thomas T . Phosphoenolpyruvate and 2-phosphoglycerate: endogenous energy source(s) for sugar accumulation by starved cells of Streptococcus lactis. J Bacteriol. 1977; 130(2):583-95. PMC: 235256. DOI: 10.1128/jb.130.2.583-595.1977. View

20.

Thomas T, Turner K, Crow V . Galactose fermentation by Streptococcus lactis and Streptococcus cremoris: pathways, products, and regulation. J Bacteriol. 1980; 144(2):672-82. PMC: 294717. DOI: 10.1128/jb.144.2.672-682.1980. View