Coding Region Deletions Associated with the Major Form of RDNA Interruption in Drosophila

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1981 Oct 10

PMID 6796939

Citations 11

Authors

P M Rae

Affiliations

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Abstract

The nucleotide sequences at and around the termini of 5 kb type 1 interruptions in three separate clones of D. melanogaster rDNA repeats have been determined, and have been compared with the sequence of the corresponding region of an insertion-free rDNA repeat. All three interrupted rDNA repeats contain a small deletion of 28S rRNA coding material at the left coding/insertion sequence junction. A second deletion was found in one of the three clones, ad other aberrations were suggested by the results of restriction enzyme digestions of unfractionated rDNA. The termini of 5 kb type 1 rDNA insertions in D. melanogaster were also compared with the corresponding regions of 28S rDNA interruptions in D. virilis: the insertion site is identical in the two species, but the termini of the two species' interruptions show no homology. I sequenced a 1.1 kb region of the 5 kb type 1 D. melanogaster rDNA interruption that covers the sequences of the 1 kb and 0.5 kb insertions. There is 98% homology between the rightmost 1 kb of the 5 kb interruption and the sequences of the shorter insertions. Data suggest that Drosophila rDNA interruptions arose as a transposable element, and that divergence had included length alterations generated by unequal crossing over.

Citing Articles

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References

Maniatis T, Jeffrey A, Kleid D . Nucleotide sequence of the rightward operator of phage lambda. Proc Natl Acad Sci U S A. 1975; 72(3):1184-8. PMC: 432491. DOI: 10.1073/pnas.72.3.1184. View

Beckingham K, White R . The ribosomal DNA of Calliphora erythrocephala; and analysis of hybrid plasmids containing ribosomal DNA. J Mol Biol. 1980; 137(4):349-73. DOI: 10.1016/0022-2836(80)90162-x. View

Smith H, Birnstiel M . A simple method for DNA restriction site mapping. Nucleic Acids Res. 1976; 3(9):2387-98. PMC: 343093. DOI: 10.1093/nar/3.9.2387. View

Glover D, Hogness D . A novel arrangement of the 18S and 28S sequences in a repeating unit of Drosophila melanogaster rDNA. Cell. 1977; 10(2):167-76. DOI: 10.1016/0092-8674(77)90212-4. View

White R, Hogness D . R loop mapping of the 18S and 28S sequences in the long and short repeating units of Drosophila melanogaster rDNA. Cell. 1977; 10(2):177-92. DOI: 10.1016/0092-8674(77)90213-6. View

Wellauer P, Dawid I . The structural organization of ribosomal DNA in Drosophila melanogaster. Cell. 1977; 10(2):193-212. DOI: 10.1016/0092-8674(77)90214-8. View

Pellegrini M, Manning J, Davidson N . Sequence arrangement of the rDNA of Drosophila melanogaster. Cell. 1977; 10(2):213-4. DOI: 10.1016/0092-8674(77)90215-x. View

Dawid I, Botchan P . Sequences homologous to ribosomal insertions occur in the Drosophila genome outside the nucleolus organizer. Proc Natl Acad Sci U S A. 1977; 74(10):4233-7. PMC: 431913. DOI: 10.1073/pnas.74.10.4233. View

Wellauer P, Dawid I, Tartof K . X and Y chromosomal ribosomal DNA of Drosophila: comparison of spacers and insertions. Cell. 1978; 14(2):269-78. DOI: 10.1016/0092-8674(78)90113-7. View

10.

Dawid I, Wellauer P, Long E . Ribosomal DNA in Drosophila melanogaster. I. Isolation and characterization of cloned fragments. J Mol Biol. 1978; 126(4):749-68. DOI: 10.1016/0022-2836(78)90018-9. View

11.

Wellauer P, Dawid I . Ribosomal DNA in Drosophila melanogaster. II. Heteroduplex mapping of cloned and uncloned rDNA. J Mol Biol. 1978; 126(4):769-82. DOI: 10.1016/0022-2836(78)90019-0. View

12.

Smith M, Leung D, Gillam S, Astell C, Montgomery D, Hall B . Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae. Cell. 1979; 16(4):753-61. DOI: 10.1016/0092-8674(79)90091-6. View

13.

Barnett T, Rae P . A 9.6 kb intervening sequence in D. virilis rDNA, and sequence homology in rDNA interruptions of diverse species of Drosophila and other diptera. Cell. 1979; 16(4):763-75. DOI: 10.1016/0092-8674(79)90092-8. View

14.

Long E, Dawid I . Expression of ribosomal DNA insertions in Drosophila melanogaster. Cell. 1979; 18(4):1185-96. DOI: 10.1016/0092-8674(79)90231-9. View

15.

Jolly D, Thomas Jr C . Nuclear RNA transcripts from Drosophila melanogaster ribosomal RNA genes containing introns. Nucleic Acids Res. 1980; 8(1):67-84. PMC: 327243. DOI: 10.1093/nar/8.1.67. View

16.

DE Wet J, Daniels D, Schroeder J, Williams B, Moore D, Blattner F . Restriction maps for twenty-one Charon vector phages. J Virol. 1980; 33(1):401-10. PMC: 288556. DOI: 10.1128/JVI.33.1.401-410.1980. View

17.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

18.

Levis R, Dunsmuir P, Rubin G . Terminal repeats of the Drosophila transposable element copia: nucleotide sequence and genomic organization. Cell. 1980; 21(2):581-8. DOI: 10.1016/0092-8674(80)90496-1. View

19.

Calos M, Miller J . Transposable elements. Cell. 1980; 20(3):579-95. DOI: 10.1016/0092-8674(80)90305-0. View

20.

Roiha H, Glover D . Chracterisation of complete type II insertions in cloned segments of ribosomal DNA from Drosophila melanogaster. J Mol Biol. 1980; 140(2):341-55. DOI: 10.1016/0022-2836(80)90110-2. View