Mycoplasma Pneumoniae Infection of Intact Guinea Pig Tracheas Cultured in a Unique Matrix-embed/perfusion System

Overview

Journal In Vitro

Specialties Anatomy & Histology
Cell Biology

Date 1981 Oct 1

PMID 6796499

Citations 7

Authors

M G Gabridge

L E Hoglund

Affiliations

Soon will be listed here.

Abstract

A new method for the in vitro culture of entire, intact tracheas from adult guinea pigs is described. Matrix-embed/perfusion (MEP) culture is based on an immobilization of the tissue in nutrient agar The tubular piece of agar-embedded organ was contained in a special perfusion block with two wells for liquid medium at either end. When incubated on a rocker platform, liquid medium flows through the trachea and supplies oxygen an nutrients. In this configuration, tracheas maintain near-normal metabolism (ATP content and dehydrogenase activity), structure (as determined by light and electron microscopy), and function (ciliary motion). Tissues could be maintained in vitro in a normal for at least 4 wk, with reduced ciliary motion and cell metabolism detectable for at least 6 wk. Agar-embedded tissues from the MEP cultures were nearly identical to those cultivated with standard tracheal ring explant techniques. Tracheas in the MEP cultures were infected with Mycoplasma pneumoniae. Attachment was neuraminidase-sensitive. Mycoplasma attachment was lowest on the epithelium along the dorsal ridge, but was uniform along the length of the trachea. Ciliostasis and cytonecrosis induced by M. pneumoniae was dose dependent. The matrix-embed/perfuse technique appears to have considerable potential for several types of in vitro studies on trachea or other tubular organs.

Citing Articles

Influenza virus infection decreases tracheal mucociliary velocity and clearance of Streptococcus pneumoniae.

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Attachment of Mycoplasma pneumoniae to tracheal monolayer outgrowths.

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Quantitation of adherence of mucoid and nonmucoid Pseudomonas aeruginosa to hamster tracheal epithelium.

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Functional and ultrastructural effects of nontypeable Haemophilus influenzae in a hamster trachea organ culture system.

Mylotte J, Stack R, Murphy T, Asirwatham J, Apicella M In Vitro Cell Dev Biol. 1985; 21(10):575-82.

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Frequency of fimbriation of nontypable Haemophilus influenzae and its ability to adhere to chinchilla and human respiratory epithelium.

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