The Chamber/Dish: an Improved Vessel for Cell and Explant Culture

Overview

Journal In Vitro

Specialties Anatomy & Histology
Cell Biology

Date 1981 Feb 1

PMID 6792053

Citations 4

Authors

M G Gabridge

Affiliations

Soon will be listed here.

Abstract

A vessel consisting of a Teflon well former, metal base plate, rubber gasket, and variable growth surface has been constructed. It is nontoxic, autoclavable, and reusable. It provides for ready access into the growth chamber, plus an optically clear lower surface for improved microscopic resolution. The vessel has been used successfully with several different eucaryotic and procaryotic cell systems. Morphology of cells and yields (as measured by total protein and ATP) are comparable to those noted with other vessels. This Chamber/Dish can accommodate almost any type of growth substrate in the form of films or sheets. It appears to have widespread application for the growth of cells and explants in vitro.

Citing Articles

Electrically conducting polymers can noninvasively control the shape and growth of mammalian cells.

Wong J, Langer R, Ingber D Proc Natl Acad Sci U S A. 1994; 91(8):3201-4.

PMID: 8159724 PMC: 43543. DOI: 10.1073/pnas.91.8.3201.

Attachment of Mycoplasma pneumoniae to tracheal monolayer outgrowths.

Gabridge M Yale J Biol Med. 1983; 56(5-6):657-63.

PMID: 6433577 PMC: 2590516.

Gaseous oxide toxicity evaluated with cell monolayers on collagen-coated, gas-permeable teflon membranes.

Gabridge M, Gladd M Environ Health Perspect. 1984; 54:347-52.

PMID: 6428874 PMC: 1568145. DOI: 10.1289/ehp.8454347.

Lymph node metastasis and cell movement: ultrastructural studies on the rat 13762 mammary carcinoma and Walker carcinoma.

Carr I, Levy M, Orr K, Bruni J Clin Exp Metastasis. 1985; 3(2):125-39.

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References

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Gabridge M, Polisky R . Intracellular levels of adenosine triphosphate in hamster trachea organ cultures exposed to Mycoplasma pneumoniae cells or membranes. In Vitro. 1977; 13(8):510-6. DOI: 10.1007/BF02615144. View