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Estimation of Energy Metabolism in Human Skeletal Muscle Homogenate As a Diagnostic Aid

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Publisher Wiley
Date 1981 Jan 1
PMID 6790863
Citations 6
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Abstract

A method is presented for the in vitro testing of the energy metabolism of human skeletal muscle. The levels of creatine phosphate, ATP, ADP and AMP, which are defined by the activity of many enzyme system, are measured after incubation of the muscle homogenate with various substrates in the presence or absence of specific inhibitors. Detection of abnormal metabolite concentrations is important for diagnosis of patients with myopathic syndromes.

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References
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Bookelman H, Trijbels J, Sengers R, Janssen A, Veerkamp J, STADHOUDERS A . Pyruvate oxidation in rat and human skeletal muscle mitochondria. Biochem Med. 1978; 20(3):395-403. DOI: 10.1016/0006-2944(78)90089-3. View