Acid-soluble Spore Proteins of Bacillus Subtilis

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1981 Jun 1

PMID 6787020

Citations 21

Authors

W C Johnson

D J Tipper

Affiliations

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Abstract

Acid-soluble spore proteins (ASSPs) comprise about 5% of the total protein of mature spores of different Bacillus subtilis strains. They consist of three abundant species, alpha, beta, and gamma, four less abundant species, and several minor species, alpha, beta, and gamma make up about 18, 18 and 36%, respectively, of the total ASSPs of strain 168, have molecular weights of 5,900, 5,9000, and 11,000, respectively, and resemble the major (A, C, and B) components of Bacillus megaterium ASSPs in several respects, including sensitivity to a specific B. megaterium spore endopeptidase. However, they have pI's of 6.58, 6.67, and 7.96, all lower than those of any of the B. megaterium ASSPs. Although strains varied in the proportions of different ASSPs, to overall patterns seen on gel electrophoresis are constant. ASSPs are located interior to the cortex, presumably in the spore cytoplasm, and are synthesized during sporulation and degraded during germination.

Citing Articles

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Genomic determinants of sporulation in Bacilli and Clostridia: towards the minimal set of sporulation-specific genes.

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Small, acid-soluble proteins as biomarkers in mass spectrometry analysis of Bacillus spores.

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New small, acid-soluble proteins unique to spores of Bacillus subtilis: identification of the coding genes and regulation and function of two of these genes.

Bagyan I, Setlow B, Setlow P J Bacteriol. 1998; 180(24):6704-12.

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ssp genes and spore osmotolerance in Bacillus thuringiensis israelensis and Bacillus sphaericus.

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