Differential Characterization of Two Leucine Aminopeptidases in Drosophila Melanogaster

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 1981 Feb 1

PMID 6784719

Citations 5

Authors

V K Walker

J H Williamson

R B Church

Affiliations

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Abstract

Two leucine aminopeptidases from Drosophila melanogaster larvae have been partially purified. The LAP A and D enzymes have similar biochemical characteristics including molecular weights of approximately equal to 280,000 daltons. Michaelis-Menten constants of approximately equal to 0.05 mM, associations with metal cofactors, and specificities toward natural and chromogenic substrates. They differ in their pH optima and spatial distributions. If the closely linked genes that code for these enzymes have resulted from a tandem gene duplication event, it is suggested that there has been subsequent evolutionary divergence. This would provide Drosophila larvae with two related, but functionally distinct enzymes.

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References

SMITHIES O . An improved procedure for starch-gel electrophoresis: further variations in the serum proteins of normal individuals. Biochem J. 1959; 71(3):585-7. PMC: 1196836. DOI: 10.1042/bj0710585. View

LEFEVRE Jr G, Green M . Genetic duplication in the white-split interval of the X chromosome in Drosophila melanogaster. Chromosoma. 1972; 36(4):391-412. DOI: 10.1007/BF00336795. View

RITOSSA F, Spiegelman S . LOCALIZATION OF DNA COMPLEMENTARY TO RIBOSOMAL RNA IN THE NUCLEOLUS ORGANIZER REGION OF DROSOPHILA MELANOGASTER. Proc Natl Acad Sci U S A. 1965; 53:737-45. PMC: 221060. DOI: 10.1073/pnas.53.4.737. View

Radola B . Thin-layer gel filtration of proteins. I. Method. J Chromatogr. 1968; 38(1):61-77. DOI: 10.1016/0021-9673(68)85009-5. View

Schabort J, Du Toii P . An aminopeptidase from Agave americana variegata--II: Aspects of enzyme kinetics, specificity, inhibition and mechanism of action. Int J Biochem. 1978; 9(3):171-7. DOI: 10.1016/0020-711x(78)90146-5. View

Ward C . Resolution of proteases in the keratinolytic larvae of the webbing clothes moth. Aust J Biol Sci. 1975; 28(1):1-23. View

Hubby J, Lewontin R . A molecular approach to the study of genic heterozygosity in natural populations. I. The number of alleles at different loci in Drosophila pseudoobscura. Genetics. 1966; 54(2):577-94. PMC: 1211185. DOI: 10.1093/genetics/54.2.577. View

Lakovaara S, Saura A . Location of enzyme loci in chromosomes of Drosophila willistoni. Experientia. 1972; 28(3):355-6. DOI: 10.1007/BF01928737. View

MAROUX S, Louvard D, Baratti J . The aminopeptidase from hog intestinal brush border. Biochim Biophys Acta. 1973; 321(1):282-95. DOI: 10.1016/0005-2744(73)90083-1. View

10.

Vesterberg O . Staining of protein zones after isoelectric focusing in polyacrylamide gels. Biochim Biophys Acta. 1971; 243(2):345-8. DOI: 10.1016/0005-2795(71)90094-8. View

11.

Gooding R, Rolseth B . Digestive processes of haematophagous insects. XI. Partial purification and some properties of six proteolytic enzymes from the tsetse fly Glossina morsitans morsitans Westwood (Diptera: Glossinidae). Can J Zool. 1976; 54(11):1950-9. DOI: 10.1139/z76-226. View

12.

Basha S, Horst M, Bazer F, Roberts R . Peptidases from pig reproductive tract: purification and properties of aminopeptidases from uterine secretions, allantoic fluid, and amniotic fluid. Arch Biochem Biophys. 1978; 185(1):174-84. DOI: 10.1016/0003-9861(78)90157-1. View

13.

Ward C . Aminopeptidases in webbing clothes moth larvae. properties and specificities of enzymes of highest electrophoretic mobility. Aust J Biol Sci. 1975; 28(5-6):447-55. DOI: 10.1071/bi9750447. View

14.

SAKAI R, Tung D, Scandalios J . Developmental genetic studies of aminopeptidases in Drosophila melanogaster. Mol Gen Genet. 1969; 105(1):24-9. DOI: 10.1007/BF00750312. View

15.

Marks N, Datta R, Lajtha A . Partial resolution of brain arylamidases and aminopeptidases. J Biol Chem. 1968; 243(11):2882-9. View

16.

Morris C . THIN-LAYER CHROMATOGRAPHY OF PROTEINS ON SEPHADEX G-100 AND G-200. J Chromatogr. 1964; 16:167-75. DOI: 10.1016/s0021-9673(01)82451-1. View

17.

Himmelhoch S . Leucine aminopeptidase: a zinc metloenzyme. Arch Biochem Biophys. 1969; 134(2):597-602. DOI: 10.1016/0003-9861(69)90322-1. View

18.

Gordon A, LOUIS L . Preparative acrylamide electrophoresis: a single gel system. Anal Biochem. 1967; 21(2):190-200. DOI: 10.1016/0003-2697(67)90180-7. View

19.

Ward C . Aminopeptidases in webbing clothes moth larvae. Properties and specificities of the enzymes of intermediate electrophoretic mobility. Biochim Biophys Acta. 1975; 410(2):361-9. DOI: 10.1016/0005-2744(75)90238-7. View

20.

Ferguson K, Wallace A . Starch-gel electrophoresis of anterior pituitary hormones. Nature. 1961; 190:629-30. DOI: 10.1038/190629a0. View