Inclusion of Xylan in a Medium for the Enumeration of Total Culturable Rumen Bacteria

Overview

Journal Appl Environ Microbiol

Specialties Environmental Health
Microbiology

Date 1978 Jun 1

PMID 677867

Citations 8

Authors

P A Henning

A E van der Walt

Affiliations

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Abstract

The influence of the inclusion of xylan in a medium for enumeration of total culturable rumen bacteria was investigated. Maximum colony numbers were obtained on a medium, GCSX-2, which contained 0.033% each glucose and cellobiose and 0.067% each soluble starch and xylan. This medium gave higher colony counts than either medium 98-5 of Bryant and Robinson (J. Dairy Sci. 44:1446-1456, 1961), medium 98-5 of Chung and Hungate (Appl. Environ. Microbiol. 32:649-652, 1976), containing an added lucerne (hemicellulose + cellulose) fiber substrate, or medium GCSX-2 with the added lucerne (hemicellulose + cellulose) fraction. The time of collection of rumen fluid influenced the colony counts on the media containing the lucerne fiber substrate but was without effect on medium GCSX-2.

Citing Articles

Examination of methods for enumerating hemicellulose-utilizing bacteria in the rumen.

Henning P Appl Environ Microbiol. 1979; 38(1):13-7.

PMID: 16345407 PMC: 243427. DOI: 10.1128/aem.38.1.13-17.1979.

Use of Congo red-polysaccharide interactions in enumeration and characterization of cellulolytic bacteria from the bovine rumen.

Teather R, Wood P Appl Environ Microbiol. 1982; 43(4):777-80.

PMID: 7081984 PMC: 241917. DOI: 10.1128/aem.43.4.777-780.1982.

Differential carbohydrate media and anaerobic replica plating techniques in delineating carbohydrate-utilizing subgroups in rumen bacterial populations.

Leedle J, Hespell R Appl Environ Microbiol. 1980; 39(4):709-19.

PMID: 6769390 PMC: 291408. DOI: 10.1128/aem.39.4.709-719.1980.

Rumen bacterial and fungal degradation of Digitaria pentzii grown with or without sulfur.

Akin D, Gordon G, HOGAN J Appl Environ Microbiol. 1983; 46(3):738-48.

PMID: 6639027 PMC: 239343. DOI: 10.1128/aem.46.3.738-748.1983.

Characterization of microbial proteolytic enzymes in the rumen.

Prins R, van Rheenen D, Vant Klooster A Antonie Van Leeuwenhoek. 1983; 49(6):585-95.

PMID: 6370133 DOI: 10.1007/BF00399852.

References

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Dehority B, Grubb J . Basal medium for the selective enumeration of rumen bacteria utilizing specific energy sources. Appl Environ Microbiol. 1976; 32(5):703-10. PMC: 170387. DOI: 10.1128/aem.32.5.703-710.1976. View

Chung K, HUNGATE R . Effect of alfalfa fiber substrate on culture counts of rumen bacteria. Appl Environ Microbiol. 1976; 32(4):649-52. PMC: 170333. DOI: 10.1128/aem.32.4.649-652.1976. View

Dehority B . Hemicellulose degradation by rumen bacteria. Fed Proc. 1973; 32(7):1819-25. View

van Gylswyk N . A comparison of two techniques for counting cellulolytic rumen bacteria. J Gen Microbiol. 1970; 60(2):191-7. DOI: 10.1099/00221287-60-2-191. View