Signal Peptides and Signal Peptidase in Drosophila Melanogaster

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 1980 Nov 1

PMID 6776130

Citations 7

Authors

M D Brennan

T G Warren

A P Mahowald

Affiliations

Soon will be listed here.

Abstract

Translation of poly(A)-containing RNA from the female fat body of Drosophila melanogaster in a rabbit reticulocyte cell-free system results in the synthesis of previtellogenin polypeptides (PVs) having higher apparent molecular weights (46,000 and 45,000) than the forms seen after an in vivo pulse labeling. However, when this RNA is translated in the presence of EDTA-stripped microsomal membranes from the dog pancreas, vitellogenin precursors are produced that, upon SDS-polyacrylamide gel electrophoresis, comigrate with the in vivo forms (apparent molecular weights, 45,000 and 44,000). These processed forms are sequestered within the microsomal lumen, as evidenced by their insensitivity to trypsin digestion. Neither processing nor sequestration occur posttranslationally. In addition, a microsomal membrane fraction derived from Drosophila embryos is able to cotranslationally process the PVs as well as a murine pre-light chain IgG. These observations support a signal-mediated mode of secretion in Drosophila, and suggest that signal sequence recognition and signal peptidase activities are conserved even between mammalian and insect systems.

Citing Articles

Biosynthesis and regulation of two vitellogenins in the fat body and ovaries of Ceratitis capitata (Diptera).

Rina M, Mintzas A Rouxs Arch Dev Biol. 2017; 197(3):167-174.

PMID: 28305561 DOI: 10.1007/BF00427920.

Drosophila signal peptidase complex member Spase12 is required for development and cell differentiation.

Haase Gilbert E, Kwak S, Chen R, Mardon G PLoS One. 2013; 8(4):e60908.

PMID: 23573290 PMC: 3616019. DOI: 10.1371/journal.pone.0060908.

Identification of a female-sterile mutation affecting yolk protein 2 in Drosophila melanogaster.

Williams J, Saunders R, Bownes M, Scott A Mol Gen Genet. 1987; 209(2):360-5.

PMID: 17191346 DOI: 10.1007/BF00329666.

The sequence and expression pattern of the Calliphora erythrocephala yolk protein A and B genes.

Martinez A, Bownes M J Mol Evol. 1994; 38(4):336-51.

PMID: 8007002 DOI: 10.1007/BF00163151.

Vitellogenin in Drosophila melanogaster: a comparison of the YPI and YPII genes and their transcription products.

Hovemann B, Galler R Nucleic Acids Res. 1982; 10(7):2261-74.

PMID: 6806773 PMC: 320608. DOI: 10.1093/nar/10.7.2261.

References

HORIBATA K, Harris A . Mouse myelomas and lymphomas in culture. Exp Cell Res. 1970; 60(1):61-77. DOI: 10.1016/0014-4827(70)90489-1. View

Bonner W, Laskey R . A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Eur J Biochem. 1974; 46(1):83-8. DOI: 10.1111/j.1432-1033.1974.tb03599.x. View

Laskey R, Mills A . Quantitative film detection of 3H and 14C in polyacrylamide gels by fluorography. Eur J Biochem. 1975; 56(2):335-41. DOI: 10.1111/j.1432-1033.1975.tb02238.x. View

Blobel G, Dobberstein B . Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J Cell Biol. 1975; 67(3):835-51. PMC: 2111658. DOI: 10.1083/jcb.67.3.835. View

Tata J . The expression of the vitellogenin gene. Cell. 1976; 9(1):1-14. DOI: 10.1016/0092-8674(76)90047-7. View

Allis C, Waring G, Mahowald A . Mass isolation of pole cells from Drosophila melanogaster. Dev Biol. 1977; 56(2):372-81. DOI: 10.1016/0012-1606(77)90277-9. View

Suchanek G, KREIL G, Hermodson M . Amino acid sequence of honeybee prepromelittin synthesized in vitro. Proc Natl Acad Sci U S A. 1978; 75(2):701-4. PMC: 411324. DOI: 10.1073/pnas.75.2.701. View

Shields D, Blobel G . Efficient cleavage and segregation of nascent presecretory proteins in a reticulocyte lysate supplemented with microsomal membranes. J Biol Chem. 1978; 253(11):3753-6. View

Wyatt G, Pan M . Insect plasma proteins. Annu Rev Biochem. 1978; 47:779-817. DOI: 10.1146/annurev.bi.47.070178.004023. View

10.

Scott M, Storti R, Pardue M, Rich A . Cell-free protein synthesis in lysates of Drosophila melanogaster cells. Biochemistry. 1979; 18(8):1588-94. DOI: 10.1021/bi00575a032. View

11.

Warren T, Mahowald A . Isolation and partial chemical characterization of the three major yolk polypeptides from Drosophila melanogaster. Dev Biol. 1979; 68(1):130-9. DOI: 10.1016/0012-1606(79)90248-3. View

12.

Waring G, Allis C, Mahowald A . Isolation of polar granules and the identification of polar granule-specific protein. Dev Biol. 1978; 66(1):197-206. DOI: 10.1016/0012-1606(78)90284-1. View

13.

Warren T, Brennan M, Mahowald A . Two processing steps in maturation of vitellogenin polypeptides in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979; 76(6):2848-52. PMC: 383706. DOI: 10.1073/pnas.76.6.2848. View

14.

Thireos G, Shea R, Kafatos F . Identification of chorion protein precursors and the mRNAs that encode them in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1979; 76(12):6279-83. PMC: 411847. DOI: 10.1073/pnas.76.12.6279. View

15.

Blobel G, Walter P, Chang C, GOLDMAN B, Erickson A, Lingappa V . Translocation of proteins across membranes: the signal hypothesis and beyond. Symp Soc Exp Biol. 1979; 33:9-36. View

16.

Spradling A, Digan M, Mahowald A, Scott M, Craig E . Two clusters of genes for major chorion proteins of Drosophila melanogaster. Cell. 1980; 19(4):905-14. DOI: 10.1016/0092-8674(80)90082-3. View

17.

DULBECCO R, Freeman G . Plaque production by the polyoma virus. Virology. 1959; 8(3):396-7. DOI: 10.1016/0042-6822(59)90043-1. View