Ultrastructural Evaluation of the Effects of 1,25-dihydroxyvitamin D3 on Bone of Thyroparathyroidectomized Rats Fed a Low-calcium Diet

Overview

Journal Am J Pathol

Publisher Elsevier

Specialty Pathology

Date 1978 Aug 1

PMID 677270

Citations 10

Authors

S E Weisbrode

C C Capen

A W Norman

Affiliations

Soon will be listed here.

Abstract

Thyroparathyroidectomized rats fed a low-calcium-normal-phosphorus diet were administered 1 or 5 units of 1,25-dihydroxyvitamin D3-(1,25-[OH]2D3) or placebo daily for 7 days. 1,25-(OH)2D3 elevated serum and urine calcium and decreased serum phosphorus. Rats given 1 unit of 1,25-(OH)2D3 had increased numbers of osteoclasts in metaphyseal trabeculae, Ultrastructurally, osteoclasts, osteoblasts, and osteocytes in rats given 1 unit of 1,25-(OH)2D3, were similar to those in rats given placebo. In rats given 5 units of 1,25-(OH)2D3, osteoclasis was markedly increased. Osteoblasts were more numerous and interpreted to be active in matrix production and mineralization. Lamellated electron-dense bodies were observed adjacent to the plasma membranes of less active osteoblasts and were interpreted to be modified matrix. Most osteocytes in rats given 5 units of 1,25-(OH)2D3 were indistinguishable from osteocytes in rats given placebo. However,the pericellular space of some osteocytes in rats given 5 units of 1,25-(OH)2D3 contained electron-dense granular deposits that were interpreted to be calcium phosphate. It is concluded that 1,25-(OH)2D3 is able to significantly elevate serum calcium independent of dietary calcium, parathyroid hormone, and calcitonin primarily by increasing ostoeclasis with minimal dependence on osteocytic osteolysis.

Citing Articles

Vitamin D and orthodontics: an insight review.

Almoammar K Clin Cosmet Investig Dent. 2018; 10:165-170.

PMID: 30104902 PMC: 6072678. DOI: 10.2147/CCIDE.S157840.

Osteoclast formation in response to intraperitoneal injection of 1 alpha-hydroxycholecalciferol in mice.

Tinkler S, Williams D, Johnson N J Anat. 1981; 133(Pt 1):91-7.

PMID: 7319902 PMC: 1167727.

Histomorphometric evaluation of the effects of intermittent 1,25-dihydroxycholecalciferol administration on cortical bone remodeling in adult dogs.

High W, Capen C, Black H Am J Pathol. 1981; 104(1):41-9.

PMID: 6894823 PMC: 1903742.

Continuous infusion of 1,25-dihydroxyvitamin D3 stimulates bone turnover in the normal young mouse.

Marie P, Travers R Calcif Tissue Int. 1983; 35(4-5):418-25.

PMID: 6688545 DOI: 10.1007/BF02405070.

24,25(OH)2D3, bone formation, and bone resorption in vitamin D-deficient, azotemic rats.

Goodman W, Baylink D, Sherrard D Calcif Tissue Int. 1984; 36(2):206-13.

PMID: 6430503 DOI: 10.1007/BF02405319.

References

CAULFIELD J, SCHRAG P . ELECTRON MICROSCOPIC STUDY OF RENAL CALCIFICATION. Am J Pathol. 1964; 44:365-81. PMC: 1907019. View

Scarpelli D . EXPERIMENTAL NEPHROCALCINOSIS. A BIOCHEMICAL AND MORPHOLOGIC STUDY. Lab Invest. 1965; 14:123-41. View

Giacomelli F, SPIRO D, Wiener J . A STUDY OF METASTATIC RENAL CALCIFICATION AT THE CELLULAR LEVEL. J Cell Biol. 1964; 22:189-206. PMC: 2106482. DOI: 10.1083/jcb.22.1.189. View

Rasmussen H, Deluca H, Arnaud C, Hawker C, VONSTEDINGK M . THE RELATIONSHIP BETWEEN VITAMIN D AND PARATHYROID HORMONE. J Clin Invest. 1963; 42:1940-6. PMC: 289482. DOI: 10.1172/JCI104880. View

Reynolds J, Pavlovitch H, BALSAN S . 1,25-Dihydroxycholecalciferol increases bone resorption in thyroparathyroidectomised mice. Calcif Tissue Res. 1976; 21(3):207-12. DOI: 10.1007/BF02547397. View

Weisbrode S, Capen C, Pendley 2nd C . Interaction of dichloromethylene diphosphonate and vitamin D on bone of thyroparathyroidectomized rats. Am J Pathol. 1977; 89(1):137-52. PMC: 2032203. View

Raisz L, Trummel C, Holick M, DeLuca H . 1,25-dihydroxycholecalciferol: a potent stimulator of bone resorption in tissue culture. Science. 1972; 175(4023):768-9. DOI: 10.1126/science.175.4023.768. View

Weisbrode S, Capen C, Nagode L . Fine structural and enzymatic evaluation of bone in thyroparathyroidectomized rats receiving various levels of vitamin D. Lab Invest. 1973; 28(1):29-37. View

Fraser D, KODICEK E . Regulation of 25-hydroxycholecalciferol-1-hydroxylase activity in kidney by parathyroid hormone. Nat New Biol. 1973; 241(110):163-6. DOI: 10.1038/newbio241163a0. View

10.

Miller S, Jee W . Ethane-1-hydroxy-1, 1-diphosphonate (EHDP). Effects on growth and modeling of the rat tibia. Calcif Tissue Res. 1975; 18(3):215-31. DOI: 10.1007/BF02546241. View

11.

DeLuca H . Parathyroid hormone as a trophic hormone for 1,25-dihydroxyvitamin D3, the metabolically active form of vitamin D. N Engl J Med. 1972; 287(5):250-1. DOI: 10.1056/NEJM197208032870511. View

12.

Garabedian M, Holick M, DeLuca H, Boyle I . Control of 25-hydroxycholecalciferol metabolism by parathyroid glands. Proc Natl Acad Sci U S A. 1972; 69(7):1673-6. PMC: 426775. DOI: 10.1073/pnas.69.7.1673. View

13.

Tanaka Y, DeLuca H . Bone mineral mobilization activity of 1,25-dihydroxycholecalciferol, a metabolite of vitamin D. Arch Biochem Biophys. 1971; 146(2):574-8. DOI: 10.1016/0003-9861(71)90163-9. View

14.

Melancon Jr M, DeLuca H . Interrelationships between thyrocalcitonin, parathyroid hormone and vitamin D: control of serum calcium in hypervitaminosis D. Endocrinology. 1969; 85(4):704-10. DOI: 10.1210/endo-85-4-704. View

15.

Frankel S, Yasumura S . Changes in the thyroidal content of thyrocalcitonin produced by vitamin D in rats. Endocrinology. 1971; 88(1):267-70. DOI: 10.1210/endo-88-1-267. View

16.

Omdahl J, DeLuca H . Regulation of vitamin D metabolism and function. Physiol Rev. 1973; 53(2):327-72. DOI: 10.1152/physrev.1973.53.2.327. View

17.

Weisbrode S, Capen C, Nagode L . Ultrastructural evaluation of the effects of vitamin D and uremia on bone in the rat. Am J Pathol. 1974; 76(2):359-76. PMC: 1910857. View

18.

Weisbrode S, Capen C, Nagode L . Influence of parathyroid hormone on ultrastructural and enzymatic changes induced by vitamin D in bone of thyroparathyroidectomized rats. Lab Invest. 1974; 30(6):786-94. View

19.

Norman A, Henry H . 1,25-Dihydroxycholecalciferol--a hormonally active form of vitamin D. Recent Prog Horm Res. 1974; 30(0):431-80. View

20.

Weisbrode S, Capen C . Ultrastructural evaluation of the interaction of glucocorticoids and vitamin D on bone cells in thyroparathyroidectomized rats. Am J Pathol. 1976; 84(3):457-68. PMC: 2032516. View