High Efficiency Temperature-sensitive Amber Suppressor Strains of Escherichia Coli K12: Construction and Characterization of Recombinant Strains with Suppressor-enhancing Mutations

Overview

Journal Mol Gen Genet

Specialties Genetics
Molecular Biology

Date 1980 Jan 1

PMID 6771486

Citations 14

Authors

M P Oeschger

G T Wiprud

Affiliations

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Abstract

A set of eight strains combining the supD43,74 ts1 suppressor gene with alleles of three suppressor-enhancing (sue) genes have been constructed and characterized. The sue mutations work cooperatively to raise suppressor activity and together raise the activity of the supD43,74-encoded suppressor 40-fold. These strains further expand the utility of the ts suppressor system by providing as much as 100% suppressor activity at temperatures at or below 20 degrees C to as little as 0.015% suppressor activity at 43 degrees C.

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References

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