6-Phosphogluconate Dehydrogenase from Drosophila Melanogaster. I. Purification and Properties of the A Isozyme

Overview

Journal Biochem Genet

Specialty Molecular Biology

Date 1980 Feb 1

PMID 6770845

Citations 5

Authors

J H Williamson

D Krochko

B W Geer

Affiliations

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Abstract

6-Phosphogluconate dehydrogenase is evident at all developmental stages of Drosophila melanogaster. The activity level is highest in early third instar larvae and declines to a lower, but relatively constant, level at all later stages of development. The enzyme is localized in the cytosolic portion of the cell. The A-isozymic form of 6-phosphogluconate dehydrogenase was purified to homogeneity and has a molecular weight of 105,000. The enzyme is a dimer consisting of subunits with molecular weights of 55,000 and 53,000. For the oxidative decarboxylation of 6-phosphogluconate the Km for substrate is 81 muM while that for NADP+ is 22.3 muM. The optimum pH for activity is 7.8 while the optimum temperature is 37 C.

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References

Geer B, Krochko D, Williamson J . Ontogeny, cell distribution, and the physiological role of NADP-malic enxyme in Drosophila melanogaster. Biochem Genet. 1979; 17(9-10):867-79. DOI: 10.1007/BF00504309. View

Geer B, BOWMAN J, Simmons J . The pentose shunt in wild-type and glucose-6-phosphate dehydrogenase deficient Drosophila melanogaster. J Exp Zool. 1974; 187(1):77-86. DOI: 10.1002/jez.1401870110. View

Kazazian Jr H . Molecular size studies on 6-phosphogluconate dehydrogenase. Nature. 1966; 212(5058):197-8. DOI: 10.1038/212197a0. View

Dyson J, DOrazio R, Hanson W . Sheep liver 6-phosphogluconate dehydrogenase: isolation procedure and effect of pH, ionic strength, and metal ions on the kinetic parameters. Arch Biochem Biophys. 1973; 154(2):623-35. DOI: 10.1016/0003-9861(73)90017-9. View

Simcox P, Dennis D . 6-Phosphogluconate Dehydrogenase Isoenzymes from the Developing Endosperm of Ricinus communis L. Plant Physiol. 1978; 62(2):287-90. PMC: 1092107. DOI: 10.1104/pp.62.2.287. View

Rippa M, Signorini M . 6-Phosphogluconate dehydrogenase from Candida utilis. Methods Enzymol. 1975; 41:237-40. DOI: 10.1016/s0076-6879(75)41054-0. View

Horie Y . Dehydrogenase in carbohydrate metabolism in larvae of the silkworm, Bombyx mori L. J Insect Physiol. 1967; 13(8):1163-75. DOI: 10.1016/0022-1910(67)90090-x. View

Gvozdev V, Gerasimova T, Kogan G, Rosovsky J . Investigations on the organization of genetic loci in Drosophila melanogaster: lethal mutations affecting 6-phosphogluconate dehydrogenase and their suppression. Mol Gen Genet. 1977; 153(2):191-8. DOI: 10.1007/BF00264735. View

Betts S, Mayer R . Purification and properties of 6-phosphogluconate dehydrogenase from rabbit mammary gland. Biochem J. 1975; 151(2):263-70. PMC: 1172355. DOI: 10.1042/bj1510263. View

10.

Rippa M, Signorini M, Pontremoli S . Purification and properties of two forms of 6-phosphogluconate dehydrogenase from Candida utilis. Eur J Biochem. 1967; 1(2):170-8. DOI: 10.1007/978-3-662-25813-2_26. View

11.

Geer B, Kamiak S, Kidd K, Nishimura R, Yemm S . Regulation of the oxidative NADP-enzyme tissue levels in Drosophila melanogaster. I. Modulation by dietary carbohydrate and lipid. J Exp Zool. 1976; 195(1):15-32. DOI: 10.1002/jez.1401950103. View

12.

Gvozdev V, Gerasimova T, Kogan G, Braslavskaya OYu . Role of the pentose phosphate pathway in metabolism of Drosophila melanogaster elucidated by mutations affecting glucose 6-phosphate and 6-phosphogluconate dehydrogenases. FEBS Lett. 1976; 64(1):85-8. DOI: 10.1016/0014-5793(76)80255-4. View

13.

Rippa M, Signorini M, Bellini T, Dallocchio F . The active site of 6-phosphogluconate dehydrogenase. A phosphate binding site and its surroundings. Arch Biochem Biophys. 1978; 189(2):516-23. DOI: 10.1016/0003-9861(78)90241-2. View

14.

BROWDER L, Williamson J . The effects of cinnamon on xanthine dehydrogenase, aldehyde oxidase, and pyridoxal oxidase activity during development in Drosophila melanogaster. Dev Biol. 1976; 53(2):241-9. DOI: 10.1016/0012-1606(76)90226-8. View

15.

Dyson J, DOrazio R . Sheep liver 6-phosphogluconate dehydrogenase. Inhibition by nucleoside phosphates and by other metabolic intermediates. J Biol Chem. 1973; 248(15):5428-35. View

16.

GLOCK G, McLean P . Further studies on the properties and assay of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase of rat liver. Biochem J. 1953; 55(3):400-8. PMC: 1269290. DOI: 10.1042/bj0550400. View

17.

Lucchesi J . Dosage compensation in Drosophila. Annu Rev Genet. 1973; 7:225-37. DOI: 10.1146/annurev.ge.07.120173.001301. View

18.

SEECOF R, Kaplan W, Futch D . Dosage compensation for enzyme activities in Drosophila melanogaster. Proc Natl Acad Sci U S A. 1969; 62(2):528-35. PMC: 277832. DOI: 10.1073/pnas.62.2.528. View

19.

BOWMAN J, Simmons J . Gene modulation in Drosophila: dosage compensation of Pgd+ and Zw+ genes. Biochem Genet. 1973; 10(4):319-31. DOI: 10.1007/BF00485987. View

20.

Pearse B, Rosemeyer M . The molecular weight and subunit structure of human erythrocyte 6-phosphogluconate dehydrogenase. Eur J Biochem. 1974; 42(1):225-35. DOI: 10.1111/j.1432-1033.1974.tb03332.x. View