Thioglycolate Medium Decreases Resistance to Bacterial Infection in Mice

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1980 Feb 1

PMID 6769800

Citations 15

Authors

L A Baker

P A Campbell

Affiliations

Soon will be listed here.

Abstract

Brewer thioglycolate medium, a bacterial culture medium which is widely used as a nonspecific in vivo macrophage stimulant, was tested for its effect on mouse resistance to bacterial infection. Mice which did or did not receive thioglycolate medium were challenged with Listeria monocytogenes. Injection of thioglycolate medium significantly decreased the ability of the host to resist infection. This decreased resistance occurred whether thioglycolate medium was injected 0 to 9 days before, or 1 to 2 h after Listeria challenge. Bacteria grew considerably better in washed adherent peritoneal cells from thioglycolate medium-injected mice than in washed adherent peritoneal cells from normal mice. Thus, thioglycolate medium may ultimately cause a decrease in macrophage function.

Citing Articles

Immunological effects of vitamin c and zinc on tilapia (Orechromis niloticus) exposed to cold water stress.

Mustafa A, Belavilas M, Hossain R, Mishu I PLoS One. 2024; 19(9):e0311078.

PMID: 39325792 PMC: 11426438. DOI: 10.1371/journal.pone.0311078.

Systemic Listeria monocytogenes infection in aged mice induces long-term neuroinflammation: the role of miR-155.

Cassidy B, Sonntag W, Leenen P, Drevets D Immun Ageing. 2022; 19(1):25.

PMID: 35614490 PMC: 9130456. DOI: 10.1186/s12979-022-00281-0.

Neuroinvasive Listeria monocytogenes infection triggers accumulation of brain CD8 tissue-resident memory T cells in a miR-155-dependent fashion.

Cassidy B, Zhang M, Sonntag W, Drevets D J Neuroinflammation. 2020; 17(1):259.

PMID: 32878636 PMC: 7466815. DOI: 10.1186/s12974-020-01929-8.

Neuroinvasive Infection Triggers IFN-Activation of Microglia and Upregulates Microglial miR-155.

Zhang M, Gillaspy A, Gipson J, Cassidy B, Nave J, Brewer M Front Immunol. 2018; 9:2751.

PMID: 30538705 PMC: 6277692. DOI: 10.3389/fimmu.2018.02751.

Alpha-galactosylceramide promotes killing of Listeria monocytogenes within the macrophage phagosome through invariant NKT-cell activation.

Emoto M, Yoshida T, Fukuda T, Kawamura I, Mitsuyama M, Kita E Infect Immun. 2010; 78(6):2667-76.

PMID: 20351146 PMC: 2876567. DOI: 10.1128/IAI.01441-09.

References

Karnovsky M, Lazdins J . Biochemical criteria for activated macrophages. J Immunol. 1978; 121(3):809-13. View

Edelson P, Zwiebel R, COHN Z . The pinocytic rate of activated macrophages. J Exp Med. 1975; 142(5):1150-64. PMC: 2189970. DOI: 10.1084/jem.142.5.1150. View

Ruco L, Meltzer M . Macrophage activation for tumor cytotoxicity: increased lymphokine responsiveness of peritoneal macrophages during acute inflammation. J Immunol. 1978; 120(3):1054-62. View

Emmerling P, Finger H, Hof H . Cell-mediated resistance to infection with Listeria monocytogenes in nude mice. Infect Immun. 1977; 15(2):382-5. PMC: 421378. DOI: 10.1128/iai.15.2.382-385.1977. View

Doe W, Henson P . Macrophage stimulation by bacterial lipopolysaccharides. I. Cytolytic effect on tumor target cells. J Exp Med. 1978; 148(2):544-56. PMC: 2184936. DOI: 10.1084/jem.148.2.544. View

Hibbs Jr J, Taintor R, Chapman Jr H, Weinberg J . Macrophage tumor killing: influence of the local environment. Science. 1977; 197(4300):279-82. DOI: 10.1126/science.327547. View

Evans R, Alexander P . Mechanism of immunologically specific killing of tumour cells by macrophages. Nature. 1972; 236(5343):168-70. DOI: 10.1038/236168a0. View

Baker L, Campbell P . Listeria monocytogenes cell walls induce decreased resistance to infection. Infect Immun. 1978; 20(1):99-107. PMC: 421557. DOI: 10.1128/iai.20.1.99-107.1978. View

Mackaness G . The influence of immunologically committed lymphoid cells on macrophage activity in vivo. J Exp Med. 1969; 129(5):973-92. PMC: 2138649. DOI: 10.1084/jem.129.5.973. View

10.

Nathan C, Karnovsky M, David J . Alterations of macrophage functions by mediators from lymphocytes. J Exp Med. 1971; 133(6):1356-76. PMC: 2138934. DOI: 10.1084/jem.133.6.1356. View

11.

Unkeless J, Gordon S, Reich E . Secretion of plasminogen activator by stimulated macrophages. J Exp Med. 1974; 139(4):834-50. PMC: 2139579. DOI: 10.1084/jem.139.4.834. View

12.

Kato N, Kato O, Nakashima I . Effect of capsular polysaccharide of Klebsiella pneumoniae on host resistance to bacterial infections. I. Induction of increased susceptibility to infections in mice. Jpn J Microbiol. 1976; 20(3):163-72. DOI: 10.1111/j.1348-0421.1976.tb00971.x. View

13.

COHN Z . Activation of mononuclear phagocytes: fact, fancy, and future. J Immunol. 1978; 121(3):813-6. View

14.

Mishell R, Dutton R . Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967; 126(3):423-42. PMC: 2138377. DOI: 10.1084/jem.126.3.423. View

15.

Nathan C, Brukner L, Silverstein S, COHN Z . Extracellular cytolysis by activated macrophages and granulocytes. I. Pharmacologic triggering of effector cells and the release of hydrogen peroxide. J Exp Med. 1979; 149(1):84-99. PMC: 2184749. DOI: 10.1084/jem.149.1.84. View

16.

Hahn H, Bierther M . Morphological changes induced by dextran sulfate 500 in mononuclear phagocytes of listeria-infected mice. Infect Immun. 1974; 10(5):1110-9. PMC: 423070. DOI: 10.1128/iai.10.5.1110-1119.1974. View

17.

Campbell P, Caldwell J, Hartman A . Use of a macrophage cytotoxicity system to show macrophage activation by Listeria monocytogenes cell wall fraction. Scand J Immunol. 1979; 10(1):1-9. DOI: 10.1111/j.1365-3083.1979.tb01327.x. View

18.

Evans R, Grant C, Cox H, Steele K, Alexander P . Thymus-derived lymphocytes produce an immunologically specific macrophage-arming factor. J Exp Med. 1972; 136(5):1318-22. PMC: 2139296. DOI: 10.1084/jem.136.5.1318. View

19.

Sword C . Mechanisms of pathogenesis in Listeria monocytogenes infection. I. Influence of iron. J Bacteriol. 1966; 92(3):536-42. PMC: 276286. DOI: 10.1128/jb.92.3.536-542.1966. View

20.

Edelson P, COHN Z . 5'-Nucleotidase activity of mouse peritoneal macrophages. I. Synthesis and degradation in resident and inflammatory populations. J Exp Med. 1976; 144(6):1581-95. PMC: 2190476. DOI: 10.1084/jem.144.6.1581. View