A New RNA Polymerase and in Vitro Transcription of the Origin of Replication from Rat Mitochondrial DNA

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1982 Dec 11

PMID 6760128

Citations 4

Authors

K Yaginuma

M Kobayashi

M Taira

K Koike

Affiliations

Soon will be listed here.

Abstract

A new RNA polymerase was found in a rat mitochondrial extract. This enzyme showed strong template preference in vitro for the supercoiled recombinant plasmid consisting of pBR322 and the D-loop region of rat mtDNA carrying the origin of heavy-strand replication. The main products synthesized by the D-loop region were two RNAs of different sizes. Both of these products were light-strand products transcribed from the region upstream from the origin of replication. This specific transcription is discussed in relation to initiation of primer RNA synthesis for heavy-strand replication of rat mtDNA.

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