DNA Synthesis in Escherichia Coli B/r After UV-irradiation

Overview

Journal Folia Microbiol (Praha)

Publisher Springer

Specialty Microbiology

Date 1982 Jan 1

PMID 6757070

Authors

H Kucerova

B VECHET

Affiliations

Soon will be listed here.

Abstract

When studying the kinetics of DNA synthesis, growth and cell division in Escherichia coli B/r after irradiation with different doses of UV-radiation (254 nm) we could demonstrate, by means of pulse incorporation of 3H-thymidine, a lag in DNA synthesis after the irradiation. The relative rate of the restored DNA synthesis (related to the number of viable cells) was higher than in the non-irradiated culture. After 3 h the rate of DNA synthesis settled at a constant value, which was identical with the control rate up to the "critical dose" of 20 J/m2. The irradiated cell population is heterogenous and contains basically two categories of cells--surviving and non-surviving. Cells of both types contribute to DNA synthesis restored after the lag period to a different extent. During the first hour after the irradiation even the nonviable portion of the population, i.e. cells that do not form colonies but are still penicillin-sensitive, is involved in the DNA synthesis.

References

Schwarz U, Asmus A, Frank H . Autolytic enzymes and cell division of Escherichia coli. J Mol Biol. 1969; 41(3):419-29. DOI: 10.1016/0022-2836(69)90285-x. View

Smith K, OLeary M . The pitfalls of measuring DNA synthesis kinetics as exemplifed in ultraciolet radiation studies. Biochim Biophys Acta. 1968; 169(2):430-8. DOI: 10.1016/0005-2787(68)90051-8. View

Helmstetter C, PIERUCCI O . Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli. J Bacteriol. 1968; 95(5):1627-33. PMC: 252187. DOI: 10.1128/jb.95.5.1627-1633.1968. View

DOUDNEY C . Ultraviolet light effects on the bacterial cell. Curr Top Microbiol Immunol. 1968; 46:116-75. DOI: 10.1007/978-3-642-46121-7_4. View

HAEFNER K, Striebeck U . Radiation-induced lethal sectoring in Escherichia coli B/r and Bs-1. Mutat Res. 1967; 4(4):399-407. DOI: 10.1016/0027-5107(67)90002-4. View

Boyle J, Swenson P . Loss of the capacity of UV-irradiated Escherichia coli B-r to grow T4D. Virology. 1971; 44(1):37-45. DOI: 10.1016/0042-6822(71)90150-4. View

DOUDNEY C . Rifampicin limitation of DNA synthesis in ultraviolet-damaged bacteria: evidence for postirradiation replication synchrony. Biochim Biophys Acta. 1973; 312(2):243-7. DOI: 10.1016/0005-2787(73)90369-9. View

Smith K . DNA synthesis in sensitive and resistant mutants of Escherichia coli B after ultraviolet irradiation. Mutat Res. 1969; 8(3):481-95. DOI: 10.1016/0027-5107(69)90065-7. View

BILLEN D, Bruns L . The dampening of DNA replication cycles after x-irradiation or ultraviolet irradiation. Biophys J. 1970; 10(6):509-18. PMC: 1367794. DOI: 10.1016/s0006-3495(70)86316-0. View

10.

Swenson P, Setlow R . Effects of ultraviolet radiation on macromolecular synthesis in Escherichia coli. J Mol Biol. 1966; 15(1):201-19. DOI: 10.1016/s0022-2836(66)80221-8. View

11.

BILLEN D . Replication of the bacterial chromosome: location of new initiation sites after irradiation. J Bacteriol. 1969; 97(3):1169-75. PMC: 249831. DOI: 10.1128/jb.97.3.1169-1175.1969. View

12.

DOUDNEY C . Deoxyribonucleic acid replication in UV-damaged bacteria revisited. Mutat Res. 1971; 12(2):121-8. DOI: 10.1016/0027-5107(71)90133-3. View

13.

Swenson P, Schenley R . Role of Pyridine Nucleotides in the Control of Respiration in Ultraviolet-Irradiated Escherichia coli B/r Cells. J Bacteriol. 1970; 104(3):1230-5. PMC: 248281. DOI: 10.1128/jb.104.3.1230-1235.1970. View

14.

Hewitt R, BILLEN D . Reorientation of chromosome replication after exposure to ultraviolet light of Escherichia coli. J Mol Biol. 1965; 13(1):40-53. DOI: 10.1016/s0022-2836(65)80078-x. View

15.

Achey P, BILLEN D . Saturation of dark repair synthesis: accumulation of strand breaks. Biophys J. 1969; 9(5):647-53. PMC: 1367745. DOI: 10.1016/S0006-3495(69)86409-X. View

16.

Barner H, COHEN S . The relation of growth to the lethal damage induced by ultraviolet irradiation in Escherichia coli. J Bacteriol. 1956; 71(2):149-57. PMC: 357757. DOI: 10.1128/jb.71.2.149-157.1956. View

17.

DOUDNEY C, Young C . Ultraviolet Light Induced Mutation and Deoxyribonucleic Acid Replication in Bacteria. Genetics. 1962; 47(9):1125-38. PMC: 1210395. DOI: 10.1093/genetics/47.9.1125. View

18.

Anderson E . Growth Requirements of Virus-Resistant Mutants of Escherichia Coli Strain "B". Proc Natl Acad Sci U S A. 1946; 32(5):120-8. PMC: 1078898. DOI: 10.1073/pnas.32.5.120. View

19.

Swenson P, Schenley R . Evidence for the control of respiration by DNA in ultraviolet-irradiated Escherichia coli B-r cells. Mutat Res. 1970; 9(5):443-53. DOI: 10.1016/0027-5107(70)90028-x. View

20.

Setlow R, Swenson P, Carrier W . THYMINE DIMERS AND INHIBITION OF DNA SYNTHESIS BY ULTRAVIOLET IRRADIATION OF CELLS. Science. 1963; 142(3598):1464-6. DOI: 10.1126/science.142.3598.1464. View