15N NMR Study of a Mixture of Uniformly Labeled TRNAs

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1982 Aug 1

PMID 6750610

Citations 1

Authors

N C Gonnella

T R Birdseye

M Nee

J D Roberts

Affiliations

Soon will be listed here.

Abstract

15N NMR spectra were taken of 15N-enriched tRNA extracted from bakers yeast; ammonium sulfate was used as a nitrogen source. The increase in the degree of denaturation of tRNA, which occurs with increase in temperature from 30 degrees C to 70 degrees C, resulted in no large changes in 15N chemical shifts at acidic and neutral pH but quite pronounced changes in proton-15N nuclear Overhauser effects.

Citing Articles

Multiple quantum two-dimensional 1H--15N nuclear magnetic resonance spectroscopy: chemical shift correlation maps for exchangeable imino protons of Escherichia coli tRNAMetf in water.

Griffey R, Poulter C, Bax A, Hawkins B, Yamaizumi Z, Nishimura S Proc Natl Acad Sci U S A. 1983; 80(19):5895-7.

PMID: 6351069 PMC: 390182. DOI: 10.1073/pnas.80.19.5895.

References

Rubin G . Preparation of RNA and ribosomes from yeast. Methods Cell Biol. 1975; 12:45-64. DOI: 10.1016/s0091-679x(08)60951-6. View

Gust D, Moon R, Roberts J . Applications of natural-abundance nitrogen-15 nuclear magnetic resonance to large biochemically important molecules. Proc Natl Acad Sci U S A. 1975; 72(12):4696-700. PMC: 388795. DOI: 10.1073/pnas.72.12.4696. View

Markowski V, Sullivan G, Roberts J . Nitrogen-15 nuclear magnetic resonance spectroscopy of some nucleosides and nucleotides. J Am Chem Soc. 1977; 99(3):714-8. DOI: 10.1021/ja00445a009. View