Neutrophil Granulocytic Cell Antigen Defined by a Monoclonal Antibody--its Distribution Within Normal Haemic and Non-haemic Tissue

Overview

Journal J Clin Pathol

Specialty Pathology

Date 1982 Sep 1

PMID 6749907

Citations 16

Authors

H W Schienle

N Stein

W Muller-Ruchholtz

Affiliations

Soon will be listed here.

Abstract

Monoclonal antibodies were raised against normal human bone marrow cells. One of the antibodies obtained, monoclonal antibody 3C4 (MA 3C4), the subject of this paper, was characterised by immunofluorescence studies with viable normal peripheral blood and bone marrow cells and by immunoperoxidase studies using paraffin sections. In bone marrow and peripheral blood MA 3C4 reacts selectively with cells of late neutrophilic granulopoiesis (myelocytes, metamyelocytes, and neutrophilic granulocytes). Cells of erythropoiesis, thrombopoiesis and lymphopoiesis are negative. In lymph node and spleen only neutrophils react with MA 3C4. In non-haemic tissue reactivity was seen with epithelial cells of a variety of different gland ducts. Thus the antigen detected by MA 3C4 can serve as a marker for neutrophil differentiation in normal haemopoiesis and as a marker for ductal epithelial cells of a variety of organs within non-haemic tissue. The antigen is formalin-resistant and can be detected in paraffin sections. The antibody thus appears to be a valuable reagent for both haematological research and for routine pathology.

Citing Articles

Expression of CD15 in normal and metaplastic Paneth cells of the digestive tract.

Ariza A, Lopez D, Castella E, Munoz C, Zujar M, Mate J J Clin Pathol. 1996; 49(6):474-7.

PMID: 8763261 PMC: 500537. DOI: 10.1136/jcp.49.6.474.

Monoclonal antibodies against differentiation antigens of myelopoiesis.

Knapp W Blut. 1982; 45(5):301-8.

PMID: 7139128 DOI: 10.1007/BF00319523.

Further evidence for the B-cell nature of hairy cells. A study using immunostaining of splenic tissue with a wide panel of monoclonal antibodies.

Pallesen G, Kerndrup G, Ellegaard J Blut. 1984; 49(5):395-403.

PMID: 6388668 DOI: 10.1007/BF00319888.

Nature of non-B, non-T lymphomas: an immunohistological study on frozen tissues using monoclonal antibodies.

Pallesen G, Beverley P, Lane E, Madsen M, Mason D, STEIN H J Clin Pathol. 1984; 37(8):911-8.

PMID: 6381546 PMC: 498891. DOI: 10.1136/jcp.37.8.911.

Characterization of two monoclonal antibodies obtained after immunization with human liver mitochondrial membrane preparations.

Billett E, Gunn B, Mayer R Biochem J. 1984; 221(3):765-76.

PMID: 6206847 PMC: 1144106. DOI: 10.1042/bj2210765.

References

Todd 3rd R, Nadler L, Schlossman S . Antigens on human monocytes identified by monoclonal antibodies. J Immunol. 1981; 126(4):1435-42. View

Civin C, Mirro J, Banquerigo M . My-1, new myeloid-specific antigen identified by a mouse monoclonal antibody. Blood. 1981; 57(5):842-5. View

Natali P, De Martino C, Quaranta V, Nicotra M, Frezza F, Pellegrino M . Expression of Ia-like antigens in normal human nonlymphoid tissues. Transplantation. 1981; 31(1):75-8. DOI: 10.1097/00007890-198101000-00017. View

Zola H, McNamara P, Thomas M, Smart I, Bradley J . The preparation and properties of monoclonal antibodies against human granulocyte membrane antigens. Br J Haematol. 1981; 48(3):481-90. DOI: 10.1111/j.1365-2141.1981.tb02740.x. View

Fleming K, McMichael A, MORTON J, Woods J, Mcgee J . Distribution of HLA class 1 antigens in normal human tissue and in mammary cancer. J Clin Pathol. 1981; 34(7):779-84. PMC: 493815. DOI: 10.1136/jcp.34.7.779. View

METZGAR R, Borowitz M, Jones N, Dowell B . Distribution of common acute lymphoblastic leukemia antigen in nonhematopoietic tissues. J Exp Med. 1981; 154(4):1249-54. PMC: 2186493. DOI: 10.1084/jem.154.4.1249. View

Lalezari P, RADEL E . Neutrophil-specific antigens: immunology and clinical significance. Semin Hematol. 1974; 11(3):281-90. View

Kohler G, Milstein C . Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975; 256(5517):495-7. DOI: 10.1038/256495a0. View

Jager G, Hoffmann-Fezer G, Rodt H, Huhn D, Thiel E, Thierfelder S . Myeloid antigens and antigen densities in mice and men. Haematol Blood Transfus. 1977; 20:109-16. DOI: 10.1007/978-3-642-66639-1_11. View

10.

Drew S, Terasaki P . Autoimmune cytotoxic granulocyte antibodies in normal persons and various diseases. Blood. 1978; 52(5):941-52. View

11.

Claas F, Langerak J, Sabbe L, van Rood J . NE1: a new neutrophil specific antigen. Tissue Antigens. 1979; 13(2):129-34. DOI: 10.1111/j.1399-0039.1979.tb01148.x. View

12.

Mepham B, Frater W, Mitchell B . The use of proteolytic enzymes to improve immunoglobulin staining by the PAP technique. Histochem J. 1979; 11(3):345-57. DOI: 10.1007/BF01005033. View

13.

Aguado M, Pujol N, Rubiol E, Tura M, Celada A . Separation of granulocytes from peripheral blood in a single step using discontinuous density gradients of Ficoll-Urografin. A comparative study with separation by dextran. J Immunol Methods. 1980; 32(1):41-50. DOI: 10.1016/0022-1759(80)90115-5. View

14.

Breard J, Reinherz E, Kung P, Goldstein G, Schlossman S . A monoclonal antibody reactive with human peripheral blood monocytes. J Immunol. 1980; 124(4):1943-8. View

15.

Winchester R, KUNKEL H . The human Ia system. Adv Immunol. 1979; 28:221-92. View

16.

Zucker-Franklin D . Ultrastructural evidence for the common origin of human mast cells and basophils. Blood. 1980; 56(3):534-40. View

17.

Verma D, Spitzer G, Zander A, Fisher R, McCredie K, Dicke K . The myeloid progenitor cell: a parallel study of subpopulations in human marrow and peripheral blood. Exp Hematol. 1980; 8(1):32-43. View

18.

Weller P, Goetzl E . The human eosinophil: roles in host defense and tissue injury. Am J Pathol. 1980; 100(3):791-820. PMC: 1903551. View

19.

Lalezari P . Organ-specific and systemic alloantigens: Interrelationships and biologic implications. Transplant Proc. 1980; 12(3 Suppl 1):12-21. View