Molecular Cloning and Regulation of Expression of the Genes for Initiation Factor 3 and Two Aminoacyl-tRNA Synthetases

Overview

Journal J Bacteriol

Publisher American Society for Microbiology

Specialty Microbiology

Date 1982 Oct 1

PMID 6749810

Citations 14

Authors

D Elseviers

P Gallagher

A Hoffman

B Weinberg

I Schwartz

Affiliations

Soon will be listed here.

Abstract

A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1-transformed NP37 overproduce initiation factor 3 and phenylalanyl-tRNA synthetase. Gene expression from pID1 was studied in vitro in a coupled transcription-translation system and in minicells. The results suggest that the genes for initiation factor 3 and phenylalanyl- and threonyl-tRNA synthetase are regulated by different mechanisms.

Citing Articles

Linkage map of Escherichia coli K-12, edition 10: the traditional map.

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Novel E. coli mutants deficient in biosynthesis of 5-methylaminomethyl-2-thiouridine.

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Genetic mapping of pheU, an Escherichia coli gene for phenylalanine tRNA.

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Molecular cloning and sequencing of pheU, a gene for Escherichia coli tRNAPhe.

Schwartz I, Klotsky R, Elseviers D, Gallagher P, Krauskopf M, Siddiqui M Nucleic Acids Res. 1983; 11(13):4379-89.

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Expression of Escherichia coli infC: identification of a promoter in an upstream thrS coding sequence.

Pramanik A, Wertheimer S, Schwartz J, Schwartz I J Bacteriol. 1986; 168(2):746-51.

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