A General Method for the Purification of Restriction Enzymes

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1978 Jul 1

PMID 673857

Citations 127

Authors

P J Greene

H L Heyneker

F Bolivar

R L Rodriguez

M C Betlach

A A Covarrubias

K Backman

D J Russel

R Tait

H W Boyer

Affiliations

Soon will be listed here.

Abstract

An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.

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References

Bolivar F, Rodriguez R, Greene P, Betlach M, Heyneker H, Boyer H . Construction and characterization of new cloning vehicles. II. A multipurpose cloning system. Gene. 1977; 2(2):95-113. View

Roberts R . Restriction endonucleases. CRC Crit Rev Biochem. 1976; 4(2):123-64. DOI: 10.3109/10409237609105456. View

Nathans D, Smith H . Restriction endonucleases in the analysis and restructuring of dna molecules. Annu Rev Biochem. 1975; 44:273-93. DOI: 10.1146/annurev.bi.44.070175.001421. View