Acyl-acyl Carrier Protein As Substrate of the Acyltransferase of Rat Liver Microsomes

Overview

Journal Lipids

Specialty Biochemistry

Date 1984 May 1

PMID 6738313

Authors

E L PUGH

M Kates

Affiliations

Soon will be listed here.

Abstract

Acyl-acyl carrier protein (acyl-ACP) can serve as well as acyl-CoA as substrate of the 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) acyltransferase of rat-liver microsomes. The product of the acylation with either thioester substrate is predominantly phosphatidylcholine (PC) (92-95%). The acyl-group transferred from either myristoyl-CoA or myristoyl-ACP is located at the C-2 position of the phospholipid (PL). The apparent Km values for the myristoyl-CoA and myristoyl-ACP were 46 microM and 63 microM, and the corresponding apparent Vmax values were 1.0 and 1.6 nmol/min/mg. The rate of acylation with the acyl-ACP was unaffected by the addition of free CoA-SH. These data suggest that acyl-CoA and acyl-ACP are transferred to 1-acyl-GPC by the same or similar enzyme systems.

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