Delayed Rectification in the Cardiac Purkinje Fiber is Not Activated by Intracellular Calcium

Overview

Journal Biophys J

Publisher Cell Press

Specialty Biophysics

Date 1984 Apr 1

PMID 6722269

Citations 11

Authors

R S Kass

Affiliations

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Abstract

This study was designed to test the hypothesis that an outward current (Ix) responsible for action potential repolarization in the cardiac Purkinje fiber is activated by intracellular calcium (Cai). Pharmacological probes were combined with the measurement of membrane current and contractile activity under voltage clamp conditions. Experiments were designed to examine properties of Ix that have previously linked activation of this current to changes in Cai. The independence of Ix from Cai was demonstrated for each case tested. Thus, the results of these experiments support the view that Ix is not a calcium-activated current.

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References

Noble D, Tsien R . Outward membrane currents activated in the plateau range of potentials in cardiac Purkinje fibres. J Physiol. 1969; 200(1):205-31. PMC: 1350425. DOI: 10.1113/jphysiol.1969.sp008689. View

Kass R, Tsien R . Multiple effects of calcium antagonists on plateau currents in cardiac Purkinje fibers. J Gen Physiol. 1975; 66(2):169-92. PMC: 2226200. DOI: 10.1085/jgp.66.2.169. View

Gelles J, Aronson R . Voltage clamp analysis of the effects of dopamine on the transmembrane ionic currents underlying the action potential of sheep cardiac Purkinje fibers. Circ Res. 1977; 40(6):561-6. DOI: 10.1161/01.res.40.6.561. View

Meech R . Calcium-dependent potassium activation in nervous tissues. Annu Rev Biophys Bioeng. 1978; 7:1-18. DOI: 10.1146/annurev.bb.07.060178.000245. View

DiFrancesco D, McNaughton P . The effects of calcium on outward membrane currents in the cardiac Purkinje fibre. J Physiol. 1979; 289:347-73. PMC: 1281374. DOI: 10.1113/jphysiol.1979.sp012741. View

Noma A, Kotake H, Irisawa H . Slow inward current and its role mediating the chronotropic effect of epinephrine in the rabbit sinoatrial node. Pflugers Arch. 1980; 388(1):1-9. DOI: 10.1007/BF00582621. View

Scheuer T, Kass R . Phenytoin reduces calcium current in the cardiac Purkinje fiber. Circ Res. 1983; 53(1):16-23. DOI: 10.1161/01.res.53.1.16. View

Brown H, DiFrancesco D . Voltage-clamp investigations of membrane currents underlying pace-maker activity in rabbit sino-atrial node. J Physiol. 1980; 308:331-51. PMC: 1274551. DOI: 10.1113/jphysiol.1980.sp013474. View

Salata J, Jalife J . Effects of dantrolene sodium on the electrophysiological properties of canine cardiac Purkinje fibers. J Pharmacol Exp Ther. 1982; 220(1):157-66. View

10.

Kass R, Wiegers S . The ionic basis of concentration-related effects of noradrenaline on the action potential of calf cardiac purkinje fibres. J Physiol. 1982; 322:541-58. PMC: 1249687. DOI: 10.1113/jphysiol.1982.sp014054. View

11.

Kass R, Scheuer T, Malloy K . Block of outward current in cardiac Purkinje fibers by injection of quaternary ammonium ions. J Gen Physiol. 1982; 79(6):1041-63. PMC: 2216454. DOI: 10.1085/jgp.79.6.1041. View

12.

Kass R . Nisoldipine: a new, more selective calcium current blocker in cardiac Purkinje fibers. J Pharmacol Exp Ther. 1982; 223(2):446-56. View

13.

Latorre R, Miller C . Conduction and selectivity in potassium channels. J Membr Biol. 1983; 71(1-2):11-30. DOI: 10.1007/BF01870671. View

14.

Kass R . An optical monitor of tension for small cardiac preparations. Biophys J. 1981; 34(1):165-70. PMC: 1327460. DOI: 10.1016/S0006-3495(81)84843-6. View