The "loss" of Centromeres from Chromosomes of Aged Women

Overview

Journal Am J Hum Genet

Publisher Cell Press

Specialty Genetics

Date 1984 Mar 1

PMID 6711561

Citations 17

Authors

Y NAKAGOME

T Abe

S Misawa

T Takeshita

K Iinuma

Affiliations

Soon will be listed here.

Abstract

Both aneuploid cells in mitosis and nondisjunction in meiosis increase with advancing age. The cause(s) of these phenomena remains unknown. Here, it was confirmed that a positive Cd-band reflects the presence of a functioning centromere while a negative reaction is indicative of its inactivation or loss. We applied the Cd-banding technique to mitotic spreads obtained from 14 aged and 13 control females (peripheral blood culture). Of 6,474 scored chromosomes from the aged women, 62(0.96%) were Cd-negative; this was the case in 12 of 3,861 (0.31%) chromosomes from the younger controls. The difference was highly significant (P less than .001). About 60% of the 62 Cd-negative chromosomes from aged women belong to the C group. Chromosomes from six of the 14 aged females were additionally examined by either the C-band or the distamycin-DAPI technique; of 2,080 chromosomes, 13 (0.63%) showed premature separation of their centromeres (control: 7/5, 080, 0.14%). Our findings suggest that in aged women, the chromosomes tend to lose their Cd-positive material and the function of the centromere.

Citing Articles

CENP-C functions in centromere assembly, the maintenance of CENP-A asymmetry and epigenetic age in Drosophila germline stem cells.

Carty B, Dattoli A, Dunleavy E PLoS Genet. 2021; 17(5):e1009247.

PMID: 34014920 PMC: 8136707. DOI: 10.1371/journal.pgen.1009247.

Repetitive Fragile Sites: Centromere Satellite DNA As a Source of Genome Instability in Human Diseases.

Black E, Giunta S Genes (Basel). 2018; 9(12).

PMID: 30544645 PMC: 6315641. DOI: 10.3390/genes9120615.

The dark side of centromeres: types, causes and consequences of structural abnormalities implicating centromeric DNA.

Barra V, Fachinetti D Nat Commun. 2018; 9(1):4340.

PMID: 30337534 PMC: 6194107. DOI: 10.1038/s41467-018-06545-y.

Centromere Structure and Function.

Bloom K, Costanzo V Prog Mol Subcell Biol. 2017; 56:515-539.

PMID: 28840251 PMC: 6535225. DOI: 10.1007/978-3-319-58592-5_21.

How unfinished business from S-phase affects mitosis and beyond.

Mankouri H, Huttner D, Hickson I EMBO J. 2013; 32(20):2661-71.

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References

Evans H . The nucleolus, virus infection, and trisomy in man. Nature. 1967; 214(5086):361-3. DOI: 10.1038/214361a0. View

Grell R . Pairing at the chromosomal level. J Cell Physiol. 1967; 70(2):Suppl:119-46. DOI: 10.1002/jcp.1040700410. View

Henderson S, Edwards R . Chiasma frequency and maternal age in mammals. Nature. 1968; 218(5136):22-8. DOI: 10.1038/218022a0. View

Mikamo K . Intrafollicular overripeness and teratologic development. Cytogenetics. 1968; 7(3):212-33. DOI: 10.1159/000129985. View

Brown W, Law P, Smith P . Sex chromosome aneuploidy and parental age. Ann Hum Genet. 1969; 33(1):1-14. DOI: 10.1111/j.1469-1809.1969.tb01623.x. View

Sumner A . A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res. 1972; 75(1):304-6. DOI: 10.1016/0014-4827(72)90558-7. View

Eiberg H . New selective Giemsa technique for human chromosomes, Cd staining. Nature. 1974; 248(5443):55. DOI: 10.1038/248055a0. View

Evans H, Ross A . Letter: Spotted centromeres in human chromosomes. Nature. 1974; 249(460):861-2. DOI: 10.1038/249861a0. View

Roos U . Letter: are centromeric dots kinetochores?. Nature. 1975; 254(5499):463. DOI: 10.1038/254463a0. View

10.

Fitzgerald P, Pickering A, Mercer J, Miethke P . Premature centromere division: a mechanism of non-disjunction causing X chromosome aneuploidy in somatic cells of man. Ann Hum Genet. 1975; 38(4):417-28. DOI: 10.1111/j.1469-1809.1975.tb00631.x. View

11.

NAKAGOME Y, TERAMURA F, Katoaka K, Hosono F . Mental retardation, malformation syndrome andpartial 7p monosomy [45, XX, tdic (7;15) (p21;p11)]. Clin Genet. 1976; 9(6):621-4. View

12.

Stene J, Fischer G, Stene E, Mikkelsen M, Petersen E . Paternal age effect in Down's syndrome. Ann Hum Genet. 1977; 40(3):299-306. DOI: 10.1111/j.1469-1809.1977.tb00194.x. View

13.

Schweizer D, Ambros P, Andrle M . Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A. Exp Cell Res. 1978; 111(2):327-32. DOI: 10.1016/0014-4827(78)90177-5. View

14.

Matsunaga E, Tonomura A, Oishi H, Kikuchi Y . Reexamination of paternal age effect in Down's syndrome. Hum Genet. 1978; 40(3):259-68. DOI: 10.1007/BF00272186. View

15.

Daniel A . Single Cd band in dicentric translocations with one suppressed centromere. Hum Genet. 1979; 48(1):85-92. DOI: 10.1007/BF00273279. View

16.

Maraschio P, Zuffardi O, Curto F . Cd bands and centromeric function in dicentric chromosomes. Hum Genet. 1980; 54(2):265-7. DOI: 10.1007/BF00278982. View

17.

Maraschio P, Zuffardi O, Bernardi F, Bozzola M, De Paoli C, Fonatsch C . Preferential maternal derivation in inv dup(15): analysis of eight new cases. Hum Genet. 1981; 57(4):345-50. DOI: 10.1007/BF00281681. View

18.

Romain D, Sullivan J, SMYTHE R, Gebbie O, Parfitt R, Chapman C . Cd branding studies in a homologous Robertsonian 13;13 translocation. J Med Genet. 1982; 19(4):306-10. PMC: 1048902. DOI: 10.1136/jmg.19.4.306. View

19.

NAKAGOME Y, Matsubara T, Fujita H . Distribution of break points in human structural rearrangements. Am J Hum Genet. 1983; 35(2):288-300. PMC: 1685530. View

20.

Trimble B, Baird P . Maternal age and Down syndrome: age-specific incidence rates by single-year intervals. Am J Med Genet. 1978; 2(1):1-5. DOI: 10.1002/ajmg.1320020102. View