Human Bronchial Explants in Long-term Culture: Establishing a Baseline for Secretion

Overview

Journal In Vitro

Specialties Anatomy & Histology
Cell Biology

Date 1984 Feb 1

PMID 6706357

Citations 2

Authors

C E Snyder

C E Nadziejko

A Herp

Affiliations

Soon will be listed here.

Abstract

A serum-free culture medium was employed to evaluate both qualitative and quantitative aspects of secreted proteins derived from human bronchial explants over a period of 26 to 50 d. It was found that protein and hexose were secreted at a reasonably constant rate during this period. Viability of explants was assessed by histological examination, attachment to scored grids of the petri dish, incorporation of labeled precursors into tissue proteins, and by fluorographic analysis of the sodium dodecyl sulfate-polyacrylamide gel pattern of the secreted material. This culture system is useful as a model for the study of secretory products and processes and how they are affected by various stimuli.

Citing Articles

Structure, biosynthesis, and function of salivary mucins.

Wu A, Csako G, Herp A Mol Cell Biochem. 1994; 137(1):39-55.

PMID: 7845377 DOI: 10.1007/BF00926038.

Hamster tracheal organ culture in serum-free media: a quantitative comparison of in vitro epithelial morphology with that of in vivo controls.

Sigler R, Jones R, Hebel J, McDowell E In Vitro Cell Dev Biol. 1987; 23(2):100-10.

PMID: 3818502 DOI: 10.1007/BF02623589.

References

Cheng P, Sherman J, Boat T, Bruce M . Quantitation of radiolabeled mucous glycoproteins secreted by tracheal explants. Anal Biochem. 1981; 117(2):301-6. DOI: 10.1016/0003-2697(81)90782-x. View

Chamberlain J . Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979; 98(1):132-5. DOI: 10.1016/0003-2697(79)90716-4. View

Adler K, Brody A, Craighead J . Studies on the mechanism of mucin secretion by cells of the porcine tracheal epithelium. Proc Soc Exp Biol Med. 1981; 166(1):96-106. DOI: 10.3181/00379727-166-41030. View

Marom Z, Shelhamer J, Kaliner M . Effects of arachidonic acid, monohydroxyeicosatetraenoic acid and prostaglandins on the release of mucous glycoproteins from human airways in vitro. J Clin Invest. 1981; 67(6):1695-702. PMC: 370746. DOI: 10.1172/jci110207. View

Herp A, Wu A, Moschera J . Current concepts of the structure and nature of mammalian salivary mucous glycoproteins. Mol Cell Biochem. 1979; 23(1):27-44. DOI: 10.1007/BF00226677. View

Barrett L, McDowell E, Frank A, Harris C, Trump B . Long-term organ culture of human bronchial epithelium. Cancer Res. 1976; 36(3):1003-10. View

Last J, Kaizu T . Mucus glycoprotein secretion by tracheal explants: effects of pollutants. Environ Health Perspect. 1980; 35:131-7. PMC: 1568460. DOI: 10.1289/ehp.8035131. View

Blondin J, Goldstein R, Franzblau C . A gelatin-specific protease from hamster lung-derived cell cultures. In Vitro. 1982; 18(1):15-23. DOI: 10.1007/BF02796381. View

Snyder C, Nadziejko C, Herp A . Isolation of bronchial mucins from cystic fibrosis sputum by use of citraconic anhydride. Carbohydr Res. 1982; 105(1):87-93. DOI: 10.1016/s0008-6215(00)81856-x. View

10.

Heckman C . Organ and species specificity of epithelial growth. In Vitro. 1983; 19(1):31-40. DOI: 10.1007/BF02617991. View

11.

Weber K, Osborn M . The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969; 244(16):4406-12. View

12.

Mazzuca M, Lhermitte M, Lafitte J, Roussel P . Use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa. J Histochem Cytochem. 1982; 30(9):956-66. DOI: 10.1177/30.9.7130674. View

13.

Schulte B, SPICER S . Light microscopic histochemical detection of sugar residues in secretory glycoproteins of rodent and human tracheal glands with lectin-horseradish peroxidase conjugates and the galactose oxidase-Schiff sequence. J Histochem Cytochem. 1983; 31(3):391-403. DOI: 10.1177/31.3.6186733. View

14.

Snyder C, Nadziejko C, Herp A . Binding of basic proteins to glycoproteins in human bronchial secretions. Int J Biochem. 1982; 14(10):895-8. DOI: 10.1016/0020-711x(82)90072-6. View

15.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

16.

Lamb D, Reid L . The tracheobronchial submucosal glands in cystic fibrosis: a qualitative and quantitative histochemical study. Br J Dis Chest. 1972; 66(4):239-47. DOI: 10.1016/0007-0971(72)90042-3. View

17.

Chopra D . Retinoid reversal of squamous metaplasia in organ cultures of tracheas derived from hamsters fed on vitamin A-deficient diet. Eur J Cancer Clin Oncol. 1983; 19(6):847-57. DOI: 10.1016/0277-5379(83)90018-4. View