Enzyme Histochemical Studies on the Purkinje Fibers of the Atrioventricular System of the Bovine and Porcine Hearts

Overview

Journal Histochem J

Specialty Biochemistry

Date 1978 Jul 1

PMID 669982

Citations 10

Authors

A E Meijer

G P Vries

Affiliations

Soon will be listed here.

Abstract

In this communication the results of applying various histochemical semipermeable membrane techniques to the localization of several enzymes in bovine and porcine heart are presented. The Purkinje fibers of the atrioventricular conducting system of the bovine heart differ from the myocardium proper in containing a greater activity of the glycolytic and gluconeogenetic enzymes--lactate dehydrogenase, glyceraldehyde-phosphate dehydrogenase, hexokinase, glucosephosphate isomerase and phosphoglucomutase, and less activity of the aerobic enzymes--NADH: nitroBT oxidoreductase and isocitrate dehydrogenase (NADP+). The metabolic reactions obtained with Purkinje fibers of the porcine heart are less pronounced. These histochemical findings are in accordance with the impression that Purkinje fibers, compared with the common myocardial fibers, have a higher rate of anaerobic metabolism and a lower rate of aerobic metabolism. The activity of the NADPH regenerating enzymes glucose-6-phosphate dehydrogenase and phosphogluconate dehydrogenase (decarboxylating), and the activity of acid hydrolases such as non-specific esterase and acid phosphatase is higher in the Purkinje fibers of both the bovine and porcine heart.

Citing Articles

The development of the Purkinje fibre system in the bovine fetal heart.

Forsgren S, Thornell L, Eriksson A Anat Embryol (Berl). 1980; 159(2):125-35.

PMID: 7447032 DOI: 10.1007/BF00304973.

Enzyme histochemical studies on the conducting system of the human heart.

Elias E, De Vries G, ELIAS R, Tigges A, Meijer A Histochem J. 1980; 12(5):577-89.

PMID: 7440254 DOI: 10.1007/BF01011931.

The histochemical demonstration of glyceraldehyde phosphate dehydrogenase activity with a semipermeable membrane technique.

de Vries C, Tigges A, Meijer A Histochem J. 1980; 12(1):119-22.

PMID: 7372501 DOI: 10.1007/BF01066542.

The development of Purkinje fibres and ordinary myocytes in the bovine fetal heart. An ultrastructural study.

Forsgren S, Thornell L Anat Embryol (Berl). 1981; 162(2):127-36.

PMID: 7283176 DOI: 10.1007/BF00306485.

Early and late changes in the metabolic pattern of the working myocardial fibres and Purkinje fibres of the human heart under ischaemic and inflammatory conditions: an enzyme histochemical study.

Elias E, ELIAS R, De Vries G, Meijer A Histochem J. 1982; 14(3):445-59.

PMID: 7118569 DOI: 10.1007/BF01011856.

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