Hyperthermia Enhances the Reactivation of Irradiated Adenovirus in HeLa Cells

Overview

Journal Br J Cancer

Specialty Oncology

Date 1984 Feb 1

PMID 6696820

Citations 1

Authors

S M Piperakis

A G McLennan

Affiliations

Soon will be listed here.

Abstract

The reactivation of U.V.-irradiated adenovirus 2 in HeLa cells is enhanced 8-9 fold if the cells are given a brief hyperthermic shock before infection. Maximum reactivation is achieved by heating for 10 min at 45.5 degrees C and with a delay of 36 h between heating and infection. The induction process requires protein synthesis only during the 3 h period immediately following heating; cycloheximide does not prevent the expression of enhanced reactivation if added to the cells after this time. Heat-enhanced reactivation exhibits properties similar in some respects to radiation-enhanced reactivation and indicates an increased capacity of the heated cells to tolerate DNA damage.

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