Isolation of Repetitive DNA Sequences from Human Chromosome 21

Overview

Journal Am J Hum Genet

Publisher Cell Press

Specialty Genetics

Date 1984 Jan 1

PMID 6695923

Citations 7

Authors

G J Graham

T J Hall

M R Cummings

Affiliations

Soon will be listed here.

Abstract

We have developed a method for the isolation of phage from the human genomic library that carry repetitive DNA sequences highly represented on specific human chromosomes. We have used this technique to select recombinants carrying inserts concentrated on chromosome 21. Five clones, representing two families of sequences, have been characterized. Members of each family show cross-homology, but the two families show no homology with each other. In all but one case, the clones do not contain members of the human Alu repeat family. Single chromosome-concentrated repetitive sequences should prove to be useful in studies of the origin, evolution, and function of repetitive DNA and in regional chromosome mapping.

Citing Articles

Chromosomal localization of several families of repetitive sequences by in situ hybridization.

Devine E, Nolin S, Houck Jr G, Jenkins E, Brown W Am J Hum Genet. 1985; 37(1):114-23.

PMID: 3976654 PMC: 1684543.

Human chromosome-specific repetitive DNA sequences: novel markers for genetic analysis.

Moyzis R, Albright K, Bartholdi M, Cram L, Deaven L, Hildebrand C Chromosoma. 1987; 95(6):375-86.

PMID: 3677921 DOI: 10.1007/BF00333988.

Translocation and amplification of an X-chromosome DNA repeat in inbred strains of mice.

Disteche C, Gandy S, Adler D Nucleic Acids Res. 1987; 15(11):4393-401.

PMID: 3588301 PMC: 340869. DOI: 10.1093/nar/15.11.4393.

Cytogenetic analysis using quantitative, high-sensitivity, fluorescence hybridization.

Pinkel D, Straume T, Gray J Proc Natl Acad Sci U S A. 1986; 83(9):2934-8.

PMID: 3458254 PMC: 323421. DOI: 10.1073/pnas.83.9.2934.

Repeated DNA sequences in the distal long arm of the human X chromosome.

Muller U, Tantravahi U, Monaco A, Stroh H, Kunkel L, LATT S Hum Genet. 1986; 74(1):24-9.

PMID: 3019869 DOI: 10.1007/BF00278780.

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