Fine Structural Nature of Delayed Neuronal Death Following Ischemia in the Gerbil Hippocampus

Overview

Journal Acta Neuropathol

Specialty Neurology

Date 1984 Jan 1

PMID 6695555

Citations 52

Authors

T Kirino

K Sano

Affiliations

Soon will be listed here.

Abstract

An unusual, delayed neuronal death (DND) has been noticed in the hippocampus of the Mongolian gerbil following brief ischemia (Kirino 1982). On day 1 following 5--10 min of ischemia, light microscopy showed the CA1 pyramidal cells unchanged. On day 2, the cells showed massive growth of membranous cytoplasmic organelles instead of overt cellular disintegration. These neurons were destroyed extensively by day 4 after ischemic insult. Following longer ischemia (20--30 min), however, the changes in the CA1 pyramidal cells appeared faster and resembled the well-characterized ischemic cell change (ICC). To further clarify the differences between ICC and DND, gerbils were submitted to transient 5--30 min ischemia. They were perfusion-fixed following a given survival period and then processed for electron microscopy. Following transient ischemia, specimens showed slow cell changes with growth of cisterns of the endoplasmic reticulum (ER). In some CA1 neurons, the cytoplasm was shrunken and darkly stained, but they also displayed accumulation of ER cisterns. Occasionally, the CA1 cells demonstrated highly shrunken dark perikarya, no different than in ICC. These results indicate that DND seems to be the typical disease process of the CA1 sector and that a severer insult makes the change faster and more similar to ICC. ICC seems to occur when the CA1 pyramidal cells are damaged so severely that they cannot react with proliferous activity.

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References

PALAY S, Billings-Gagliardi S, Chan-Palay V . Neuronal perikarya with dispersed, single ribosomes in the visual cortex of Macaca mulatta. J Cell Biol. 1974; 63(3):1074-89. PMC: 2109373. DOI: 10.1083/jcb.63.3.1074. View

Schluter G . Ultrastructural observations on cell necrosis during formation of the neural tube in mouse embryos. Z Anat Entwicklungsgesch. 1973; 141(3):251-64. DOI: 10.1007/BF00519046. View

Sotelo C, PALAY S . Altered axons and axon terminals in the lateral vestibular nucleus of the rat. Possible example of axonal remodeling. Lab Invest. 1971; 25(6):653-71. View

Brown A, Levy D, Kublik M, Harrow J, Plum F, BRIERLEY J . Selective chromatolysis of neurons in the gerbil brain: a possible consequence of "epileptic" activity produced by common carotid artery occlusion. Ann Neurol. 1979; 5(2):127-38. DOI: 10.1002/ana.410050206. View

Ito U, Spatz M, Walker Jr J, KLATZO I . Experimental cerebral ischemia in mongolian gerbils. I. Light microscopic observations. Acta Neuropathol. 1975; 32(3):209-23. DOI: 10.1007/BF00696570. View

Kirino T . Delayed neuronal death in the gerbil hippocampus following ischemia. Brain Res. 1982; 239(1):57-69. DOI: 10.1016/0006-8993(82)90833-2. View

McGEE-RUSSELL S, Brown A, BRIERLEY J . A combined light and electron microscope study of early anoxic-ischaemic cell change in rat brain. Brain Res. 1970; 20(2):193-200. DOI: 10.1016/0006-8993(70)90288-x. View

MUNRO H, Roel L, Wurtman R . Inhibition of brain protein synthesis by doses of L-DOPA that disaggregate brain polyribosomes. J Neural Transm. 1973; 34(4):321-3. DOI: 10.1007/BF01242753. View

BUBIS J, Fujimoto T, Ito U, Mrsulja B, Spatz M, KLATZO I . Experimental cerebral ischemia in Mongolian gerbils. v. Ultrastructural changes in H3 sector of the hippocampus. Acta Neuropathol. 1976; 36(3):285-94. DOI: 10.1007/BF00685372. View

10.

Oppenheim R . Cell death of motoneurons in the chick embryo spinal cord. I. A light and electron microscopic study of naturally occurring and induced cell loss during development. J Comp Neurol. 1978; 177(1):33-57. DOI: 10.1002/cne.901770105. View

11.

Pulsinelli W, BRIERLEY J, Plum F . Temporal profile of neuronal damage in a model of transient forebrain ischemia. Ann Neurol. 1982; 11(5):491-8. DOI: 10.1002/ana.410110509. View

12.

JOHNSON Jr J . The occurrence of dark neurons in the normal and deafferentated lateral vestibular nucleus in the rat: observations by light and electron microscopy. Acta Neuropathol. 1975; 31(2):117-27. DOI: 10.1007/BF00688146. View

13.

Kleihues P, Hossmann K . Protein synthesis in the cat brain after prolonged cerebral ischemia. Brain Res. 1971; 35(2):409-18. DOI: 10.1016/0006-8993(71)90484-7. View

14.

Brown A, BRIERLEY J . Anoxic-ischaemic cell change in rat brain light microscopic and fine-structural observations. J Neurol Sci. 1972; 16(1):59-84. DOI: 10.1016/0022-510x(72)90102-5. View

15.

Suzuki K, Zagoren J . Effect of the hypocholesterolemic drug AY9944 on developing central nervous system of rats: alteration of endoplasmic reticulum in oligodendroglia. J Neurocytol. 1973; 2(4):369-81. DOI: 10.1007/BF01103795. View

16.

JENKINS L, Povlishock J, Lewelt W, Miller J, Becker D . The role of postischemic recirculation in the development of ischemic neuronal injury following complete cerebral ischemia. Acta Neuropathol. 1981; 55(3):205-20. DOI: 10.1007/BF00691320. View

17.

OConnor T, WYTTENBACH C . Cell death in the embryonic chick spinal cord. J Cell Biol. 1974; 60(2):448-59. PMC: 2109163. DOI: 10.1083/jcb.60.2.448. View

18.

Kirino T, Sano K . Selective vulnerability in the gerbil hippocampus following transient ischemia. Acta Neuropathol. 1984; 62(3):201-8. DOI: 10.1007/BF00691853. View