Evaluation of PPLO, A7B, E, and NYC Agar Media for the Isolation of Ureaplasma Urealyticum and Mycoplasma Species from the Genital Tract

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 1984 Jan 1

PMID 6690468

Citations 5

Authors

D M Yajko

E Balston

D Wood

R L Sweet

W K Hadley

Affiliations

Soon will be listed here.

Abstract

Four agar media (PPLO, NYC, A7B, and E) which are commonly used for the isolation of urogenital tract Mycoplasma species and Ureaplasma urealyticum were compared by culturing swabs of the endocervix of 334 pregnant women on all four media. To permit growth of both Mycoplasma and U. urealyticum, selective ingredients were omitted from the media tested. A7B and E agar were both satisfactory for the isolation of Mycoplasma species, recovering 92 and 82%, respectively, of all Mycoplasma species isolated. Only A7B agar was satisfactory for U. urealyticum isolation, recovering 96% of all isolates. Several modifications of PPLO, NYC, and E agar failed to significantly improve recovery of U. urealyticum on these media. A7B agar was clearly superior to all other media tested in terms of recovery rate, typical appearance of colonies, and ease of reading. A7B can be used for the isolation of both U. urealyticum and Mycoplasma species from urogenital sites.

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References

Toth A, Lesser M, Brooks C, Labriola D . Subsequent pregnancies among 161 couples treated for T-mycoplasma genital-tract infection. N Engl J Med. 1983; 308(9):505-7. DOI: 10.1056/NEJM198303033080907. View

SHEPARD M, LUNCEFORD C . EFFECT OF PH ON HUMAN MYCOPLASMA STRAINS. J Bacteriol. 1965; 89:265-70. PMC: 305502. DOI: 10.1128/jb.89.2.265-270.1965. View

Braun P, Klein J, Lee Y, KASS E . Methodologic investigations and prevalence of genital mycoplasmas in pregnancy. J Infect Dis. 1970; 121(4):391-400. DOI: 10.1093/infdis/121.4.391. View

Mardh P, Westrom L . Tubal and cervical cultures in acute salpingitis with special reference to Mycoplasma hominis and T-strain mycoplasmas. Br J Vener Dis. 1970; 46(3):179-86. PMC: 1048060. View

Braun P, Lee Y, Klein J, Marcy S, Klein T, Charles D . Birth weight and genital mycoplasmas in pregnancy. N Engl J Med. 1971; 284(4):167-71. DOI: 10.1056/NEJM197101282840401. View

Rottem S, Pfendt E, Hayflick L . Sterol requirements of T-strain mycoplasmas. J Bacteriol. 1971; 105(1):323-30. PMC: 248358. DOI: 10.1128/jb.105.1.323-330.1971. View

Gnarpe H, Friberg J . Mycoplasma and human reproductive failure. I. The occurrence of different Mycoplasmas in couples with reproductive failure. Am J Obstet Gynecol. 1972; 114(6):727-31. View

Faur Y, Weisburd M, Wilson M, May P . A new medium for the isolation of pathogenic Neisseria (NYC medium). I. Formulation and comparisons with standard media. Health Lab Sci. 1973; 10(2):44-54. View

Faur Y, Weisburd M, Wilson M . A new medium for the isolation of pathogenic Neisseria (NYC medium). II. Effect of amphotericin B and trimethoprim lactate on selectivity. Health Lab Sci. 1973; 10(2):55-60. View

10.

Faur Y, Weisburd M, Wlson M, May P . NYC medium for simultaneous isolation of Neisseria gonorrhoeae, large-colony mycoplasmas, and T-mycoplasmas. Appl Microbiol. 1974; 27(6):1041-5. PMC: 380205. DOI: 10.1128/am.27.6.1041-1045.1974. View

11.

SHEPARD M, LUNCEFORD C . Differential agar medium (A7) for identification of Ureaplasma urealyticum (human T mycoplasmas) in primary cultures of clinical material. J Clin Microbiol. 1976; 3(6):613-25. PMC: 274365. DOI: 10.1128/jcm.3.6.613-625.1976. View

12.

Stray-Pedersen B, Eng J, Reikvam T . Uterine T-mycoplasma colonization in reproductive failure. Am J Obstet Gynecol. 1978; 130(3):307-11. View

13.

KUNDSIN R, Parreno A, Poulin S . Significance of appropriate techniques and media for isolation and identification of Ureaplasma urealyticum from clinical specimens. J Clin Microbiol. 1978; 8(4):445-53. PMC: 275269. DOI: 10.1128/jcm.8.4.445-453.1978. View

14.

SHEPARD M, Combs R . Enhancement of Ureaplasma urealyticum growth on a differential agar medium (A7B) by a polyamine, putrescine. J Clin Microbiol. 1979; 10(6):931-3. PMC: 273297. DOI: 10.1128/jcm.10.6.931-933.1979. View

15.

Embree J, Krause V, Embil J, MacDonald S . Placental infection with Mycoplasma homonis and Ureaplasma urealyticum: clinical correlation. Obstet Gynecol. 1980; 56(4):475-81. View

16.

KUNDSIN R, DRISCOLL S, Pelletier P . Ureaplasma urealyticum incriminated in perinatal morbidity and mortality. Science. 1981; 213(4506):474-5. DOI: 10.1126/science.7244646. View

17.

Leland D, Lapworth M, Jones R, French M . Comparative evaluation of media for isolation of ureaplasma urealyticum and genital mycoplasma species. J Clin Microbiol. 1982; 16(4):709-14. PMC: 272451. DOI: 10.1128/jcm.16.4.709-714.1982. View

18.

Cassell G, Younger J, Brown M, Blackwell R, Davis J, Marriott P . Microbiologic study of infertile women at the time of diagnostic laparoscopy. Association of Ureaplasma urealyticum with a defined subpopulation. N Engl J Med. 1983; 308(9):502-5. DOI: 10.1056/NEJM198303033080906. View

19.

SHEPARD M . Nongonococcal urethritis associated with human strains of "T" mycoplasmas. JAMA. 1970; 211(8):1335-40. View