An Improved Colony Hybridization Method with Significantly Increased Sensitivity for Detection of Single Genes

Overview

Journal Plasmid

Date 1983 Nov 1

PMID 6657780

Citations 124

Authors

R Maas

Affiliations

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Abstract

By introducing a simple modification of existing methods, colony hybridization has been used to detect single copy genes coding for a heat-stable enterotoxin in wild-type strains isolated from patients with diarrhea. The modification described in this communication results in an approximately 100-fold increase in sensitivity, probably by increasing the total denatured plasmid DNA fixed to the paper.

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