In Vitro and in Vivo Complementation of Streptococcus Mutans Mutants Defective in Adherence

Overview

Journal Infect Immun

Publisher American Society for Microbiology

Specialty Allergy & Immunology

Date 1983 Nov 1

PMID 6642643

Citations 9

Authors

S Larrimore

H Murchison

T Shiota

S M Michalek

R Curtiss 3rd

Affiliations

Soon will be listed here.

Abstract

Previous studies have shown that adherence-defective mutants of Streptococcus mutans PS14, serotype c, can be grouped into several different phenotypic groups. In this study a method was developed to test for complementation between pairs of nonadhering mutants which possess different genotypic defects. Mutant strains UAB95 and a spectinomycin-resistant derivative of UAB95 (UAB516) were found to exhibit increased levels of adherence when grown together with UAB230 in media containing sucrose as compared to the adherence of each strain grown separately. An increase in caries was also observed in gnotobiotic rats mixedly infected with the two mutants as compared to either strain alone. Tests revealed that UAB95 produced more water-insoluble glucan than its parent strain but had a defect in glucan binding. UAB230 was found to produce levels of a defective glucan that could not be bound by mutant or wild-type cells. Our results suggest that UAB95 produces a water-insoluble glucan which is bound by UAB230, thus allowing complementation for adherence and caries production.

Citing Articles

A novel phosphotransferase system of Streptococcus mutans is responsible for transport of carbohydrates with α-1,3 linkage.

Ajdic D, Chen Z Mol Oral Microbiol. 2012; 28(2):114-28.

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Streptococcus mutans fructosyltransferase (ftf) and glucosyltransferase (gtfBC) operon fusion strains in continuous culture.

Wexler D, Hudson M, Burne R Infect Immun. 1993; 61(4):1259-67.

PMID: 8454329 PMC: 281356. DOI: 10.1128/iai.61.4.1259-1267.1993.

Cloning and DNA sequencing of the dextranase inhibitor gene (dei) from Streptococcus sobrinus.

Sun J, Wanda S, Camilli A, Curtiss 3rd R J Bacteriol. 1994; 176(23):7213-22.

PMID: 7961493 PMC: 197109. DOI: 10.1128/jb.176.23.7213-7222.1994.

Overproduction of a dextranase inhibitor by Streptococcus sobrinus mutants.

Wanda S, Camilli A, Murchison H, Curtiss 3rd R J Bacteriol. 1994; 176(23):7206-12.

PMID: 7961492 PMC: 197108. DOI: 10.1128/jb.176.23.7206-7212.1994.

In vitro inhibition of adherence of Streptococcus mutans strains by nonadherent mutants of S. mutans 6715.

Murchison H, Larrimore S, Curtiss 3rd R Infect Immun. 1985; 50(3):826-32.

PMID: 4066034 PMC: 261155. DOI: 10.1128/iai.50.3.826-832.1985.

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