Two Proteins Associated with Cerebellar Hypoplasia in Jaundiced Gunn Rats

Overview

Journal Neurochem Res

Specialties Chemistry
Neurology

Date 1983 Jun 1

PMID 6621772

Citations 1

Authors

S Aono

H Sato

R Semba

S Kashiwamata

Affiliations

Soon will be listed here.

Abstract

Two cerebellar proteins with apparent molecular weights of 250,000 (GR-250) and 50,000 (GR-50) are closely associated with cerebellar hypoplasia in jaundiced homozygous Gunn rats. These proteins, found in Gunn rat cerebellum (4-60 days of age) and cerebrum as well as staggerer mouse cerebellum, were studied with electrophoretic techniques. After 8 days of life, GR-250 decreased and GR-50 increased in the homozygous Gunn rat cerebellum. The pI's of GR-250 and GR-50 were 4.7-5.8 and 4.6-4.9, respectively, and the former protein was shown to bind to Concanavalin A. A comparative study between cerebella of Gunn rats and staggerer mice revealed that GR-250 and P400, a protein generally thought to be characteristic of the Purkinje cells, were identical. Evidence was also obtained showing that GR-250 was present in the Gunn rat cerebrum. GR-50 was not detectable in the staggerer mouse cerebellum but instead, a protein (MW 47,000) was found to be increased in the mutant mouse cerebellum.

Citing Articles

Improved separation at low temperature of glycoproteins by Con A-Sepharose affinity chromatography in the presence of sodium dodecyl sulfate (SDS).

Aono S, Sato H, Semba R, Kashiwamata S Experientia. 1985; 41(8):1084-7.

PMID: 4018235 DOI: 10.1007/BF01952156.

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