Mouse Rosette Positive B Cells Fail to Synthesize Immunoglobulin Following Incubation with Pokeweed Mitogen

Overview

Journal Clin Exp Immunol

Publisher Oxford University Press

Specialty Allergy & Immunology

Date 1983 Dec 1

PMID 6606517

Citations 1

Authors

F R Davey

A S Kurec

H V Lamberson

Affiliations

Soon will be listed here.

Abstract

Mouse erythrocytes form spontaneous rosettes with a population of B lymphocytes from normal individuals and from patients with B cell chronic lymphocytic leukaemia (CLL). Since lymphocytes from patients with CLL respond poorly to pokeweed mitogen (PWM), we have compared mouse rosette positive (MR+), mouse rosette negative (MR-), and unfractionated B lymphocytes from normal individuals in their response to PWM. Mononuclear cells were fractionated into B, MR+ and MR- cell populations and then combined in 1:1 proportions with mitomycin-C treated T cells in culture media. Lymphocyte co-cultures were incubated for up to 10 days in the presence of PWM. Supernatant immunoglobulin (SIg) levels, percentage intracytoplasmic immunoglobulin (ICIg), and proliferative responses were determined. MR+ cells alone failed to produce significant levels of SIg (P less than 0.025) or percentages of ICIg positive cells This decreased synthesis of immunoglobulin by MR+ cells occurred in the presence of adequate T cells, macrophages and a satisfactory proliferative response.

Citing Articles

Phenotype of chronic lymphocytic leukemia (CLL) B-cells. B-CLL cells express the Leu-8 antigen.

Cafaro A, Napolitano M, Zoli V, Pacilli L, Mezzaroma I, DE LAURENZI A Blut. 1987; 54(1):43-9.

PMID: 3099866 DOI: 10.1007/BF00326026.

References

Yam L, Li C, CROSBY W . Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971; 55(3):283-90. DOI: 10.1093/ajcp/55.3.283. View

Papamichail M, Brown J, HOLBOROW E . Immunoglobulins on the surface of human lymphocytes. Lancet. 1971; 2(7729):850-2. DOI: 10.1016/s0140-6736(71)90224-8. View

Dickler H, KUNKEL H . Interaction of aggregated -globulin with B lymphocytes. J Exp Med. 1972; 136(1):191-6. PMC: 2139185. DOI: 10.1084/jem.136.1.191. View

Wybran J, Carr M, Fudenberg H . The human rosette-forming cell as a marker of a population of thymus-derived cells. J Clin Invest. 1972; 51(10):2537-43. PMC: 332950. DOI: 10.1172/JCI107069. View

Gronowicz E, Coutinho A . Functional analysis of B cell heterogeneity. Transplant Rev. 1975; 24:3-40. DOI: 10.1111/j.1600-065x.1975.tb00164.x. View

Catovsky D, Papamichail M, Okos A, Miliani E, HOLBOROW E . Formation of mouse red cell rosettes by "hairy" cells. Biomedicine. 1975; 23(3):81-4. View

Catovsky D, Cherchi M, Okos A, Hegde U, GALTON D . Mouse red-cell rosettes in B-lymphoproliferative disorders. Br J Haematol. 1976; 33(2):173-7. DOI: 10.1111/j.1365-2141.1976.tb03528.x. View

Gronowicz E, Coutinho A, Melchers F . A plaque assay for all cells secreting Ig of a given type or class. Eur J Immunol. 1976; 6(8):588-90. DOI: 10.1002/eji.1830060812. View

Koziner B, Filippa D, Mertelsmann R, Gupta S, Clarkson B, GOOD R . Characterization of malignant lymphomas in leukemic phase by multiple differentiation markers of mononuclear cells. Correlations with clinical features and conventional morphology. Am J Med. 1977; 63(4):556-67. DOI: 10.1016/0002-9343(77)90201-7. View

10.

Rosenberg S, Lipsky P . Monocyte dependence of pokeweed mitogen-induced differentiation of immunoglobulin-secreting cells from human peripheral blood mononuclear cells. J Immunol. 1979; 122(3):926-31. View

11.

Foa R, Catovsky D, Brozovic M, Marsh G, Ooyirilangkumaran T, Cherchi M . Clinical staging and immunological findings in chronic lymphocytic leukemia. Cancer. 1979; 44(2):483-7. DOI: 10.1002/1097-0142(197908)44:2<483::aid-cncr2820440217>3.0.co;2-z. View

12.

Davey F, Dock N, Wolos J . Studies of lymphocyte proliferation in hairy cell leukaemia: activity in mixed lymphocyte reaction and responses to mitogens. Br J Haematol. 1980; 45(1):29-39. DOI: 10.1111/j.1365-2141.1980.tb03808.x. View

13.

Robert K . Induction of monoclonal antibody synthesis in malignant human B cells by polyclonal B cell activators. Relationship between B cell subsets and prognosis. Immunol Rev. 1979; 48:123-43. DOI: 10.1111/j.1600-065x.1979.tb00301.x. View

14.

Burns G, Cawley J . Spontaneous mouse erythrocyte-rosette formation: correlation with surface immunoglobulin phenotype in hairy-cell leukaemia. Clin Exp Immunol. 1980; 39(1):83-9. PMC: 1537939. View

15.

Dock N, Davey F . Peripheral blood monocytes in the autologous mixed lymphocyte reaction. Clin Immunol Immunopathol. 1980; 17(1):123-31. DOI: 10.1016/0090-1229(80)90080-x. View

16.

Koziner B, Kempin S, Passe S, Gee T, GOOD R, Clarkson B . Characterization of B-cell leukemias: a tentative immunomorphological scheme. Blood. 1980; 56(5):815-23. View

17.

Goldberg J, McGuire L, Dock N, Williams W, Davey F . Purification of human peripheral blood colony forming cells (CFUC). Exp Hematol. 1980; 8(9):1086-93. View

18.

Ault K, Towle M . Human B lymphocyte subsets. I. IgG-bearing B cell response to pokeweed mitogen. J Exp Med. 1981; 153(2):339-51. PMC: 2186092. DOI: 10.1084/jem.153.2.339. View

19.

Catovsky D, Wechsler A, Cherchi M . Characterization of B-cell leukemias: a tentative immunomorphological scheme. Blood. 1981; 58(2):410-1. View

20.

Lucivero G, Lawton A, Cooper M . Rosette formation with mouse erythrocytes defines a population of human B lymphocytes unresponsive to pokeweed mitogen. Clin Exp Immunol. 1981; 45(1):185-90. PMC: 1537242. View