Similarity in Effects of Na+ Gradients and Membrane Potentials on D-glucose Transport By, and Phlorizin Binding To, Vesicles Derived from Brush Borders of Rattit Intestinal Mucosal Cells

Overview

Journal J Membr Biol

Date 1978 May 3

PMID 660646

Citations 20

Authors

G Toggenburger

M Kessler

A Rothstein

G Semenza

C Tannenbaum

Affiliations

Soon will be listed here.

Abstract

Both the presence of sodium and of an electrical potential difference across the membrane have been found to be necessary in order to achieve optimal D-glucose-protectable phlorizin binding to brush border membranes from rabbit small intestine. The effect of delta approximately muNa on phlorizin binding shows a close similarity to that on D-glucose transport, confirming that phlorizin is indeed bound to the D-glucose transporting protein. Possible modulations of binding by a transmembrane potential are discussed on the basis of some models.

Citing Articles

Kinetic mechanisms of inhibitor binding: relevance to the fast-acting slow-binding paradigm.

Falk S, Oulianova N, Berteloot A Biophys J. 1999; 77(1):173-88.

PMID: 10388748 PMC: 1300320. DOI: 10.1016/S0006-3495(99)76880-3.

A method for determining the unitary functional capacity of cloned channels and transporters expressed in Xenopus laevis oocytes.

Zampighi G, Kreman M, Boorer K, Loo D, Bezanilla F, Chandy G J Membr Biol. 1995; 148(1):65-78.

PMID: 8558603 DOI: 10.1007/BF00234157.

Function and presumed molecular structure of Na(+)-D-glucose cotransport systems.

Koepsell H, Spangenberg J J Membr Biol. 1994; 138(1):1-11.

PMID: 8189427 DOI: 10.1007/BF00211064.

Ethanol-induced inhibition of glucose transport across the isolated brush-border membrane of hamster jejunum.

Dinda P, Beck I Dig Dis Sci. 1981; 26(1):23-32.

PMID: 7460705 DOI: 10.1007/BF01307972.

Transepithelial transport in cell culture: stoichiometry of Na/phlorizin binding and Na/D-glucose cotransport. A two-step, two sodium model of binding and translocation.

Misfeldt D, Sanders M J Membr Biol. 1982; 70(3):191-8.

PMID: 7186940 DOI: 10.1007/BF01870562.

References

Chesney R, SACKTOR B, Rowen R . The binding of D-glucose to the isolated luminal membrane of the renal proximal tubule. J Biol Chem. 1973; 248(6):2182-91. View

Vick H, DIEDRICH D, Baumann K . Reevaluation of renal tubular glucose transport inhibition by phlorizin analogs. Am J Physiol. 1973; 224(3):552-7. DOI: 10.1152/ajplegacy.1973.224.3.552. View

Lefevre P, Marshall J . The atachment of phloretin and analogues to human erythrocytes in connection with inhibition of sugar transport. J Biol Chem. 1959; 234:3022-6. View

Glossmann H, Neville Jr D . Phlorizin receptors in isolated kidney brush border membranes. J Biol Chem. 1972; 247(23):7779-89. View

Bode F, Baumann K, Frasch W, KINNE R . [Binding of phlorhizin to the brushborder fraction of rat kidney]. Pflugers Arch. 1970; 315(1):53-65. DOI: 10.1007/BF00587237. View

Honegger P, Gershon E . Further evidence for the multiplicity of carriers for free glucalogues in hamster small intestine. Biochim Biophys Acta. 1974; 352(1):127-34. DOI: 10.1016/0005-2736(74)90185-0. View

Schultz S, Curran P . Coupled transport of sodium and organic solutes. Physiol Rev. 1970; 50(4):637-718. DOI: 10.1152/physrev.1970.50.4.637. View

DIEDRICH D . Is phloretin the sugar transport inhibitor in intestine?. Arch Biochem Biophys. 1968; 127(1):803-12. DOI: 10.1016/0003-9861(68)90292-0. View

DIEDRICH D . On the absence of phlorizin hydrolase in renal brush border membranes. Arch Biochem Biophys. 1972; 153(1):155-61. DOI: 10.1016/0003-9861(72)90431-6. View

10.

Flagg J, Wilson T . A protonmotive force as the source of energy for galactoside transport in energy depleted Escherichia coli. J Membr Biol. 1977; 31(3):233-55. DOI: 10.1007/BF01869407. View

11.

Kessler M, Tannenbaum V, Tannenbaum C . A simple apparatus for performing short-time (1--2 seconds) uptake measurements in small volumes; its application to D-glucose transport studies in brush border vesicles from rabbit jejunum and ileum. Biochim Biophys Acta. 1978; 509(2):348-59. DOI: 10.1016/0005-2736(78)90053-6. View

12.

West I, Mitchell P . Stoicheiometry of lactose-H+ symport across the plasma membrane of Escherichia coli. Biochem J. 1973; 132(3):587-92. PMC: 1177623. DOI: 10.1042/bj1320587. View

13.

Alvarado F . Hypothesis for the interaction of phlorizin and phloretin with membrane carriers for sugars. Biochim Biophys Acta. 1967; 135(3):483-95. DOI: 10.1016/0005-2736(67)90038-7. View

14.

Jennings M, Solomon A . Interaction between phloretin and the red blood cell membrane. J Gen Physiol. 1976; 67(4):381-97. PMC: 2214918. DOI: 10.1085/jgp.67.4.381. View

15.

CRANE R . Hypothesis for mechanism of intestinal active transport of sugars. Fed Proc. 1962; 21:891-5. View

16.

Hopfer U, Nelson K, Perrotto J, Isselbacher K . Glucose transport in isolated brush border membrane from rat small intestine. J Biol Chem. 1973; 248(1):25-32. View

17.

GOLDMAN D . POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANES. J Gen Physiol. 2009; 27(1):37-60. PMC: 2142582. DOI: 10.1085/jgp.27.1.37. View

18.

LYON I . Studies on transmural potentials in vitro in relation to intestinal absorption. IV. Phlorizin-sugar interactions in rat gut. Biochim Biophys Acta. 1967; 135(3):496-506. DOI: 10.1016/0005-2736(67)90039-9. View

19.

Bihler I, Hawkins K, CRANE R . Studies on the mechanism of intestinal absorption of sugars. VI. The specificity and other properties of Na ion-dependent entrance of sugars into intestinal tissue under anaerobic conditions, in vitro. Biochim Biophys Acta. 1962; 59:94-102. DOI: 10.1016/0006-3002(62)90700-x. View

20.

Liedtke C, Hopfer U . Anion transport in brush border membranes isolated from rat small intestine. Biochem Biophys Res Commun. 1976; 76(2):579-85. DOI: 10.1016/0006-291x(77)90763-x. View