Inhibition of Lactate Dehydrogenase in Cultured SIRC Cells by Cigarette Smoke

Overview

Journal Histochemistry

Specialty Biochemistry

Date 1978 May 16

PMID 659264

Authors

N Fleming

H Walt

G S KISTLER

Affiliations

Soon will be listed here.

Abstract

SIRC cell monolayer cultures were exposed to whole smoke from a mid tar and nicotine level research cigarette (ASFC, 72 puffs), or from a high tar and nicotine level reference cigarette (Kentucky 2RI, 48 puffs) over a period of 65 days. The activity and distribution of lactate dehydrogenase (LDH) in the cells were investigated, and the electrophoretic characteristics of its isozymes studied. Cell morphology was examined by light microscopy and by transmission- and scanning electron microscopy. LDH activity was reduced by exposure to smoke from both cigarette types, the greater inhibitory effect being produced by that of the Kentucky cigarette. In addition, cells exposed to this high tar and nicotine smoke displayed intramitochondrial granules which were larger and more numerous than those found in cells exposed to the mid tar and nicotine smoke, or in the control cells. It is speculated that cation accumulation in the mitochondria may be involved in the observed inhibition of LDH activity.

References

Styles J . DNA content of cultured mammalian cells exposed to smoke and smoke fractions from cigarettes containing tobacco or NSM, a tobacco substitute. Br J Exp Pathol. 1976; 57(3):286-95. PMC: 2041076. View

Fleming N, KISTLER G . Morphology, histochemistry and isozymes of monkey kidney cells during long-term exposure to cigarette smoke. Acta Histochem. 1977; 60(1):132-45. DOI: 10.1016/S0065-1281(77)80030-5. View

Davies P, KISTLER G, Leuchtenberger C, Leuchtenberger R . Ultrastructural studies on cells of hamster lung cultures after chronic exposure to whole smoke or the gas vapour phase of cigarettes. Beitr Pathol. 1975; 155(2):168-80. DOI: 10.1016/s0005-8165(75)80190-9. View

Leuchtenberger C, Leuchtenberger R, Schneider A . Effects of marijuana and tobacco smoke on human lung physiology. Nature. 1973; 241(5385):137-9. DOI: 10.1038/241137a0. View

Rhim J, HUEBNER R . In vitro transformation assay of major fractions of cigarette smoke condensate (CSC) in mammalian cell lines. Proc Soc Exp Biol Med. 1973; 142(3):1003-7. DOI: 10.3181/00379727-142-37162. View

Greenawalt J, ROSSI C, Lehninger A . EFFECT OF ACTIVE ACCUMULATION OF CALCIUM AND PHOSPHATE IONS ON THE STRUCTURE OF RAT LIVER MITOCHONDRIA. J Cell Biol. 1964; 23:21-38. PMC: 2106507. DOI: 10.1083/jcb.23.1.21. View

Davies P, KISTLER G . The assessment of tobacco smoke toxicity in tissue and organ culture: an in vitro arrangement for exposure under defined conditions. Experientia. 1974; 30(4):436-8. DOI: 10.1007/BF01921709. View

DIPAOLO J, Nelson R, Donovan P . Morphological, oncogenic, and karyological characteristics of Syrian hamster embryo cells transformed in vitro by carcinogenic polycyclic hydrocarbons. Cancer Res. 1971; 31(8):1118-27. View

Inui N, Takayama S . Effect of cigarette tar upon tissue culture cells. Neoplastic transformation of hamster lung cells by tobacco tar in tissue culture. Br J Cancer. 1971; 25(3):574-83. PMC: 2008761. DOI: 10.1038/bjc.1971.73. View

10.

Evans C, DIPAOLO J . Neoplastic transformation of guinea pig fetal cells in culture induced by chemical carcinogens. Cancer Res. 1975; 35(4):1035-44. View

11.

Kistler G, SCHERLE W, Hauswirth A . [Improved method for the concentration and processing of free cells from minimal volumes of body fluids, using membrane filtration]. Experientia. 1970; 26(3):331-5. DOI: 10.1007/BF01900131. View

12.

Leuchtenberger C, Leuchtenberger R, Zbinden I . Gas vapour phase constituents and SH reactivity of cigarette smoke influence lung cultures. Nature. 1974; 247(5442):565-7. DOI: 10.1038/247565a0. View

13.

Leuchtenberger C, Leuchtenberger R, Ritter U, Inui N . Effects of marijuana and tobacco smoke on DNA and chromosomal complement in human lung explants. Nature. 1973; 242(5397):403-4. DOI: 10.1038/242403a0. View

14.

LOWRY O, ROSEBROUGH N, FARR A, RANDALL R . Protein measurement with the Folin phenol reagent. J Biol Chem. 1951; 193(1):265-75. View

15.

Benedict W, Rucker N, Faust J, Kouri R . Malignant transformation of mouse cells by cigarette smoke condensate. Cancer Res. 1975; 35(3):857-60. View

16.

Vasington F, Murphy J . Ca ion uptake by rat kidney mitochondria and its dependence on respiration and phosphorylation. J Biol Chem. 1962; 237:2670-7. View

17.

Holt P, Roberts L, Keast D . DNA synthesis in cell cultures following repeated exposure to fresh cigarette smoke. Experientia. 1975; 31(1):109-10. DOI: 10.1007/BF01924706. View