Actin-like Sequences Are Present on Human X and Y Chromosomes

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 1984 Aug 1

PMID 6592095

Citations 10

Authors

R Heilig

A Hanauer

K H Grzeschik

J L Mandel

Affiliations

Soon will be listed here.

Abstract

The human genome contains greater than 20 actin-related sequences, six of which at least are expressed as protein. We have shown by blot hybridization the presence of actin-like sequences on both the X and the Y chromosomes. These sequences can be detected in HindIII digests of genomic DNA, using as probe cDNA clones corresponding to human alpha skeletal actin or to a hamster (beta or gamma) cytoskeletal actin; they show more homology to the latter probe. The actin probes also detect a polymorphic DNA fragment showing autosomal inheritance with a frequency for the major allele of 0.55 in the population studied. The X-linked actin sequence has been assigned to a centromeric region between Xp11 and Xq11 by hybridization to DNAs from a panel of human-mouse hybrid cell lines, and thus lies outside the postulated region of homology between the X and Y chromosomes. The Y-linked actin sequence can serve as a marker to analyse anomalies of sex determination or of gametogenesis in man. It was found in all XY males studied but was absent from the genomic DNA of four unrelated 'XX male' subjects and two XX hermaphrodites. This shows that the region of chromosome Y which contains the actin sequence is not translocated onto the X chromosome (or onto autosomes) in these patients.

Citing Articles

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Extensive DNA sequence homologies between the human Y and the long arm of the X chromosome.

Geldwerth D, Bishop C, Guellaen G, Koenig M, Vergnaud G, Mandel J EMBO J. 1985; 4(7):1739-43.

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The human Y chromosome.

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Regional assignment of Y-linked DNA probes by deletion mapping and their homology with X-chromosome and autosomal sequences.

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The role of somatic cell genetics in human gene mapping.

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