Occurrence of Selenium-containing TRNAs in Mouse Leukemia Cells

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 May 1

PMID 6587339

Citations 11

Authors

W M Ching

Affiliations

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Abstract

Selenium incorporation into the polynucleotide structures of tRNAs has been documented in several microorganisms. In the present study, selenium-containing species were isolated from bulk tRNA preparations from 75Se-labeled mouse leukemia cells. The major 75Se-labeled species was similar in size and exhibited the same sensitivity to ribonuclease as did Escherichia coli tRNAs. The chromatographic properties of the intact major selenium-containing tRNA species indicated it to be very hydrophobic in character. The selenium component that is unstable at neutral-to-alkaline pH but is relatively stable at acid pH is not an esterified selenoamino acid. HPLC analysis of enzymic digests of the major selenium-containing species detected selenium-containing hydrophobic products (probably selenonucleosides ). These properties strongly suggest that the selenium in the mouse leukemia-cell tRNAs is present in the form of a selenium-modified nucleoside.

Citing Articles

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Selenomodification of tRNA in archaea requires a bipartite rhodanese enzyme.

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