Schwann Cell Expression of a Major Myelin Glycoprotein in the Absence of Myelin Assembly

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Mar 1

PMID 6584919

Citations 4

Authors

J F Poduslo

C T Berg

P J Dyck

Affiliations

Soon will be listed here.

Abstract

Quiescent Schwann cells in the distal segment of the permanently transected nerve produced basal levels of the major myelin glycoprotein, P0, in the absence of myelin assembly. Low levels of P0 could be detected at 35 days after transection by autoradiographic analysis of radioiodinated lectin binding after protein separation by high-resolution sodium dodecyl sulfate pore gradient electrophoresis and by fluorographic analysis after electrophoresis of [3H]fucose- and [3H]mannose-labeled glycoproteins after incorporation into endoneurial slices. Immunoreactivity to P0 in the transected nerve could also be demonstrated with antisera against P0 as evaluated by direct "immune overlay" after electrophoresis. These results indicate that the requirement for continuing signals from appropriate axons to make detectable amounts of myelin-specific proteins and glycolipids is not absolute. Schwann cells, therefore, like oligodendrocytes, can synthesize myelin components in the absence of neuronal influence, although information from neuronal elements probably is required for myelin assembly by Schwann cells and for myelin compaction by oligodendrocytes.

Citing Articles

Lysosomal delivery of the major myelin glycoprotein in the absence of myelin assembly: posttranslational regulation of the level of expression by Schwann cells.

Brunden K, Poduslo J J Cell Biol. 1987; 104(3):661-9.

PMID: 2434515 PMC: 2114530. DOI: 10.1083/jcb.104.3.661.

Emergence of three myelin proteins in oligodendrocytes cultured without neurons.

Dubois-Dalcq M, Behar T, Hudson L, Lazzarini R J Cell Biol. 1986; 102(2):384-92.

PMID: 2418030 PMC: 2114066. DOI: 10.1083/jcb.102.2.384.

Differentiation-specific regulation of Schwann cell expression of the major myelin glycoprotein.

Poduslo J, Windebank A Proc Natl Acad Sci U S A. 1985; 82(17):5987-91.

PMID: 2412226 PMC: 390679. DOI: 10.1073/pnas.82.17.5987.

Myelin sheath survival after guanethidine-induced axonal degeneration.

Kidd G, Heath J, Trapp B, Dunkley P J Cell Biol. 1992; 116(2):395-403.

PMID: 1730762 PMC: 2289291. DOI: 10.1083/jcb.116.2.395.

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