Stimulation of Human Granulocyte Function by Monoclonal Antibody WEM-G1

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1984 Mar 1

PMID 6584915

Citations 8

Authors

A F Lopez

M A Vadas

Affiliations

Soon will be listed here.

Abstract

From a panel of monoclonal antibodies (MAb) produced against purified human neutrophils, MAb WEM-G1 was selected for its ability to stimulate granulocyte function as assessed by the capacity to kill antibody-coated tumor target cells. MAb WEM-G1 bound to neutrophils and eosinophils and not to monocytes, lymphocytes, or erythrocytes, and thus identified a granulocyte differentiation antigen. It enhanced killing by both neutrophils and eosinophils in a dose-dependent fashion, with a range of effector-to-target ratios and dilutions of anti-target cell antibody. The effect of MAb WEM-G1 was additive with that of colony-stimulating factor (CSF-alpha). Similarly, the ability of neutrophils to exhibit enhanced cytotoxicity in the presence of WEM-G1 was increased by preincubation of neutrophils with CSF-alpha. Immunoprecipitation analysis showed that WEM-G1 identified a cell-surface protein on human neutrophils of approximate molecular weight 110,000. It is suggested that this structure is involved in regulation of the function of human granulocytes.

Citing Articles

Plasma membrane antigens on light density and activated human blood eosinophils.

Tai P, Bakes D, Barkans J, Spry C Clin Exp Immunol. 1985; 60(2):427-36.

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Eosinophilic disorders affecting the myocardium and endocardium: a review.

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New properties and roles for eosinophils in disease: discussion paper.

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Selective enhancement of the expression of granulocyte functional antigens 1 and 2 on human neutrophils.

Vadas M, Lopez A, Williamson D Proc Natl Acad Sci U S A. 1985; 82(8):2503-7.

PMID: 3887404 PMC: 397587. DOI: 10.1073/pnas.82.8.2503.

Monoclonal antibodies to human eosinophil plasma membrane antigens enhance the secretion of eosinophil cationic protein.

Tai P, Capron M, Bakes D, Barkans J, Spry C Clin Exp Immunol. 1986; 63(3):728-37.

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